Role of charge properties of bacterial envelope in bactericidal action of human group IIA phospholipase A2 against Staphylococcus aureus
Role of charge properties of bacterial envelope in bactericidal action of human group IIA phospholipase A2 against Staphylococcus aureus
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DOI：
10.1074/jbc.m205104200
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发表时间：
2002-12-06
影响因子：
4.8
通讯作者：
Weiss, JP
中科院分区：
文献类型：
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作者：
Koprivnjak, T;Peschel, A;Weiss, JP
哺乳动物IIA族磷脂酶A（2）（PLA（2））有效杀死金黄色葡萄球菌。这些PLA（2）的高度阳离子性质对于Ca 2+非依赖性结合和细胞壁渗透是重要的，这是膜磷脂的Ca 2+依赖性降解和细菌杀伤的先决条件。为了进一步描述细菌包膜的电荷特性，其在IIA族PLA（2）抗S.在金黄色葡萄球菌中，我们检查了阻止细胞壁（dltA）和细胞膜（mprF）聚阴离子的特异性修饰的突变的影响。与亲本菌株相比，同基因dltA（-）细菌对PLA（2）的敏感性高出30 - 100倍，而mprF（-）细菌对PLA（2）的敏感性高出30 - 100倍。
Mammalian Group IIA phospholipases A(2) (PLA(2)) potently kill Staphylococcus aureus. Highly cationic properties of these PLA(2) are important for Ca2+-independent binding and cell wall penetration, prerequisites for Ca2+- dependent degradation of membrane phospholipids and bacterial killing. To further delineate charge properties of the bacterial envelope important in Group IIA PLA(2) action against S. aureus, we examined the effects of mutations that prevent specific modifications of cell wall (dltA) and cell membrane (mprF) polyanions. In comparison to the parent strain, isogenic dltA(-) bacteria are similar to30-100x more sensitive to PLA(2), whereas mprF(-) bacteria are