EXPRESSION OF THE 3 INFLUENZA-VIRUS POLYMERASE PROTEINS IN A SINGLE CELL ALLOWS GROWTH COMPLEMENTATION OF VIRAL MUTANTS
EXPRESSION OF THE 3 INFLUENZA-VIRUS POLYMERASE PROTEINS IN A SINGLE CELL ALLOWS GROWTH COMPLEMENTATION OF VIRAL MUTANTS
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DOI：
10.1073/pnas.83.8.2709
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发表时间：
1986-04-01
影响因子：
11.1
通讯作者：
PALESE, P
中科院分区：
文献类型：
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作者：
KRYSTAL, M;RUAN, L;PALESE, P
构建了源自鼠 C127 细胞的转化细胞系，表达流感病毒 RNA 依赖性 RNA 聚合酶蛋白（PA、PB1 和 PB2）。测试了仅表达一种或全部三种蛋白质的细胞系补充在不允许的温度下孵育的温度敏感病毒突变体的能力。分离出表达所有三种聚合酶基因并补充 PB2 温度敏感突变体在非允许温度下生长的两个细胞系。其中一个品系还补充了 PA 温度敏感突变体。在这两个细胞系中获得的病毒滴度比相应温度敏感突变体在C127细胞中在不允许的温度下生长时获得的病毒滴度高12倍至1000倍。
Transformed cell lines derived from murine C127 cells were constructed that express the influenza virus RNA-dependent RNA polymerase proteins (PA, PB1, and PB2). Cell lines that express only one or all three of the proteins were tested for their ability to complement temperature-sensitive viral mutants incubated at the nonpermissive temperature. Two cell lines were isolated that express all three polymerase genes and complement the growth of PB2 temperature-sensitive mutants at the nonpermissive temperature. One of these lines also complemented PA temperature-sensitive mutants. The viral titers obtained in these two cell lines were 12-fold to 1000-fold higher than the viral titers obtained upon growth of the corresponding temperature-sensitive mutant in C127 cells at the nonpermissive temperature.