Pericardial mesoderm generates a population of coronary smooth muscle cells migrating into the heart along with ingrowth of the epicardial organ
Pericardial mesoderm generates a population of coronary smooth muscle cells migrating into the heart along with ingrowth of the epicardial organ
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DOI：
10.1006/dbio.1996.0068
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发表时间：
1996-03-15
影响因子：
2.7
通讯作者：
Gourdie, RG
中科院分区：
文献类型：
--
作者：
Mikawa, T;Gourdie, RG
冠状动脉的血管平滑肌细胞与近端主动脉的血管平滑肌细胞的区别在于许多结构和功能标准，这些标准可能包括增加的动脉粥样硬化转化倾向。目前，平滑肌亚群之间这种变异的来源尚不确定。虽然近端主动脉的平滑肌被认为是来自神经嵴，冠状动脉血管平滑肌的起源仍然是未知的。我们之前已经证明，冠状动脉血管系统的前体在心外膜开始包裹心肌的同一天进入管状心脏，并且冠状动脉血管是通过这些迁移前体的向内生长而不是通过主动脉的向外生长形成的（Mikawa和Fischman，1992）。为了研究冠状动脉平滑肌细胞的起源，用活体染料（DiI）或编码β-半乳糖苷酶的复制缺陷型逆转录病毒标记心外膜细胞产生的心外膜器官。细胞谱系标记是通过直接靶向卵内心外膜中假定的血管发生细胞或标记体外解剖的心外膜细胞，然后移植到阶段匹配的宿主胚胎中来实现的。在冠状动脉血管发生过程中的标记细胞的监测表明，前心外膜衍生的细胞纳入三个血管相关的人口，冠状动脉平滑肌，血管周围结缔组织和内皮细胞。免疫共聚焦显微镜鉴定了心外膜器官内的内皮细胞和平滑肌细胞群。结果表明：（1）心外膜包含冠状平滑肌细胞的祖细胞群，其随着心外膜的向内生长沿着迁移到心脏中，和（2）在迁移之前，建立冠状平滑肌谱系。(C)出版社：Academic Press，Inc.
The vascular smooth muscle cells of coronary arteries are distinguished from those of the proximal aorta by a number of structural and functional criteria which may include an increased propensity for atherosclerotic transformation. At present, the source of this variation between smooth muscle subpopulations is uncertain. Whilst smooth muscle of the proximal aorta is thought to be derived from neural crest, the origin of coronary vascular smooth muscle remains uncharacterized. We have previously shown that precursors of the coronary vasculature enter the tubular heart on the same day as the epicardial mantle starts to envelop the myocardium and that coronary vessels form by ingrowth of these migratory precursors and not by outgrowth from the aorta (Mikawa and Fischman, 1992). To study the origin of coronary smooth muscle cells, the proepicardial organ, from which epicardial cells arise, was tagged with either a vital dye (DiI) or replication-defective retroviruses encoding beta-galactosidase. Cellular lineage marking was achieved by either direct targeting of putative vasculogenic cells in the proepicardium in ovo or tagging dissected proepicardial cells in vitro followed by transplantation to stage-matched host embryos. Monitoring of tagged cells during coronary vasculogenesis indicate incorporation of proepicardial-derived cells into three vessel-associated populations; coronary mooth muscle, perivascular connective tissue, and endothelial cells. Immunoconfocal microscopy identified both endothelial and smooth muscle cell populations within the proepicardial organ. The results demonstrate that: (1) the proepicardium contains a progenitor population of coronary smooth muscle cells that migrates into the heart along with ingrowth of the epicardium and (2) prior to the migration, the coronary smooth muscle lineage is established. (C) 1996 Academic Press, Inc.