RELATIONSHIP BETWEEN ENZYME-ACTIVITIES INVOLVED IN OXYGEN-METABOLISM AND OXYGEN TOLERANCE IN BLACK-PIGMENTED BACTEROIDES
RELATIONSHIP BETWEEN ENZYME-ACTIVITIES INVOLVED IN OXYGEN-METABOLISM AND OXYGEN TOLERANCE IN BLACK-PIGMENTED BACTEROIDES
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DOI：
10.1177/00220345880670090901
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发表时间：
1988-09-01
影响因子：
7.6
通讯作者：
TSUNEMITSU, A
中科院分区：
文献类型：
--
作者：
AMANO, A;TAMAGAWA, H;TSUNEMITSU, A
本研究研究了黑色素拟杆菌中参与氧代谢的酶活性与氧耐受性的关系。所有菌株均具有NADH氧化酶和超氧化物歧化酶的活性，而过氧化氢酶和过氧化物酶的活性在无细胞提取物中未检测到。NADH氧化酶和超氧化物歧化酶的活性与氧耐受性有较高的相关性。超氧化物歧化酶活性与氧耐受性的相关性高于NADH氧化酶活性。在测试的物种中，牙龈拟杆菌显示出最高的酶活性，并且在氧气存在下最耐受。此外，耐氧菌株牙龈拟杆菌381的曝气过程中，这两种酶的活性均有所增加，而氧敏感菌株牙齿拟杆菌ATCC 33185的曝气过程中，这两种酶的活性则没有增加。这些结果表明，超氧化物歧化酶和NADH氧化酶可能在保护黑色色素拟杆菌免受氧毒性作用中起重要作用。
In this study, the relationship between enzyme activities involved in oxygen metabolism and the degrees of oxygen tolerance in black-pigmented Bacteroides was investigated. All strains of Bacteroides tested possessed the activities of NADH oxidase and superoxide dismutase, whereas the activites of catalase and peroxidases were not detected in the cell-free extracts. There were relatively high correlations between oxygen tolerance and activity of either NADH oxidase or superoxide dismutase. The activity of superoxide dismutase showed a higher correlation with oxygen tolerance than with that of NADH oxidase. Among the species tested, Bacteroides-gingivalis showed the highest activities of both the enzymes and was the most tolerant in the presence of oxygen. Furthermore, the activities of these two enzymes increased during aeration of the oxygen-tolerant strain Bacteroides gingivalis 381, but not in the oxygen-sensitive strain Bacteroides-denticola ATCC 33185. These results suggest that superoxide dismutase and NADH oxidase might be important for protection of black-pigmented Bacteroides against the toxic effect of oxygen.