Establishment and characterization of the rhabdomyosarcoma cell line designated NUTOS derived from the human tongue sarcoma: Special reference to the susceptibility of anti-cancer drugs
Establishment and characterization of the rhabdomyosarcoma cell line designated NUTOS derived from the human tongue sarcoma: Special reference to the susceptibility of anti-cancer drugs
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DOI：
10.1111/j.1749-0774.2010.00086.x
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发表时间：
2010-01-01
期刊：
影响因子：
4.3
通讯作者：
Mataga, Izumi
中科院分区：
文献类型：
--
作者：
Suzuki, Minako;Tominaga, Noriko;Mataga, Izumi
从一名17岁日本妇女的舌头收集原发腺泡型横纹肌肉瘤（RMS）肿瘤组织，并用于成功建立横纹肌肉瘤细胞系，命名为NUTOS。染色体分布显示NUTOS细胞系为染色体易位的超四倍体。细胞在补充有15%胎牛血清、0.1%非必需氨基酸溶液（NEAA）、50 μ g链霉素、50 U/mL青霉素和0.25 μ g /mL两性霉素B的Dulbecco改良Eagle培养基/F12中生长。NUTOS的形状包括小纺锤体、大纺锤体和长而厚的多核细胞。所有三种细胞类型都用抗结蛋白抗体免疫染色，所述抗结蛋白抗体是中等大小肌丝的标记蛋白。此外，免疫细胞化学染色显示细胞被抗MyoD、肌细胞生成素、α-肌节肌动蛋白、肌球蛋白和肌钙蛋白T免疫染色阳性。仅在小梭形细胞中观察到有丝分裂象。这些细胞在细胞顶端的外侧部分彼此结合。随后，这些细胞生长成大的多核细胞。在大梭形细胞和大多核细胞中均观察到自主收缩（约20次/min）。NUTOS细胞将血清中的血清素掺入生长培养基中。NUTOS细胞移植在裸鼠皮下组织中的组织学观察显示出与舌原发性横纹肌肉瘤相似的特征。抗癌药物的敏感性试验显示，NUTOS细胞对顺铂、紫杉醇和多西他赛呈抗药性，但对阿霉素不敏感。
Primary alveolar type of rhabdomyosarcoma (RMS) tumor tissue was collected from the tongue of a 17-year-old Japanese woman and used to successfully establish a rhabdomyosarcoma cell line, which has been designated NUTOS. The chromosomal distribution revealed that the NUTOS cell line was hyper-tetraploid with chromosomal translocation. The cells were grown in Dulbecco's modified eagle medium/F12 supplemented with 15% fetal bovine serum, 0.1% non-essential amino acids solution (NEAA), 50 mu g of streptomycin, 50 U/mL of penicillin and 0.25 mu g /mL of Fungizone. The NUTOS shapes included small spindles, large spindles and long, thick multinucleated cells. All three cell types were immunostained with anti-desmin antibody, which is a marker protein for middle sized myofilaments. Furthermore, immunocytochemical staining revealed that the cells were positively immunostained with anti-MyoD, myogenin, alpha-sarcomeric actin, myosin and troponin T. Mitotic figures were only observed in the small spindle cells. These cells were coadunated with each other at the lateral portion of the apex of the cells. Subsequently, these cells grew into large multinucleated cells. Autonomic contractions (approximately 20 times/min) were observed in both the large spindle cells and the large multinucleated cells. NUTOS cells incorporated serotonin from the serum in the growth medium. Histopathological observations of the NUTOS cell grafts in the subcutis of nude mice exhibited characteristics similar to those seen for the primary rhabdomyosarcoma of the tongue. Susceptibility tests for the anti-cancer drugs revealed that NUTOS cells were susceptive to cisplatin, paclitaxel, and docetaxel, but not to adriacin.