Upregulation of CD19+CD24hiCD38hi regulatory B cells is associated with a reduced risk of acute lung injury in elderly pneumonia patients
Upregulation of CD19+CD24hiCD38hi regulatory B cells is associated with a reduced risk of acute lung injury in elderly pneumonia patients
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DOI：
10.1007/s11739-015-1377-3
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发表时间：
2016-04-01
影响因子：
4.6
通讯作者：
Bai, Jianwen
中科院分区：
文献类型：
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作者：
Song, Haihan;Xi, Jianjun;Bai, Jianwen
急性肺损伤（Acute lung injury， ALI）是老年肺炎患者的常见并发症，病程进展迅速，死亡率高。由于急性呼吸道感染的治疗选择仅限于支持性护理，因此许多研究的重点是确定急性呼吸道感染发展风险较高的肺炎患者。在这里，我们从免疫学的角度通过检查CD19(+)CD24(hi)CD38(hi) B细胞来解决这个问题，CD19(+)CD24(hi) B细胞是急性和慢性炎症的重要参与者。我们发现老年肺炎患者的CD19(+)CD24(hi)CD38(hi) B细胞频率高于健康个体。这种B细胞群可能表达更高水平的IL-10，已被证明通过IL-10依赖机制抑制CD4(+) T细胞介导的促炎细胞因子干扰素γ (IFNg)和肿瘤坏死因子α (TNFa)的产生。我们还观察到外周血中CD19(+)CD24(hi)CD38(hi) B细胞的频率与CD4(+)CD25(+)Foxp3(+)Tregs的频率呈正相关。此外，与CD19(+)CD24(hi)CD38(hi) B细胞的抗炎作用一致，我们发现后来发展为ALI的肺炎患者CD19(+)CD24(hi)CD38(hi) B细胞水平降低。总之，我们的研究结果表明，肺炎患者的CD19(+)CD24(hi)CD38(hi) B细胞在体内具有调节功能，并与降低ALI风险相关。
Acute lung injury (ALI) is a common complication in elderly pneumonia patients who have a rapid progression, and is accompanied by a high mortality rate. Because the treatment options of ALI are limited to supportive care, identifying pneumonia patients who are at higher risk of ALI development is the emphasis of many studies. Here, we approach this problem from an immunological perspective by examining CD19(+)CD24(hi)CD38(hi) B cells, an important participant in acute and chronic inflammation. We find that elderly pneumonia patients have elevated CD19(+)CD24(hi)CD38(hi) B cell frequency compared to healthy individuals. This B cell population may express a higher level of IL-10, which has been was shown to suppress CD4(+) T cell-mediated proinflammatory cytokine interferon gamma (IFNg) and tumor necrosis factor alpha (TNFa) production, through an IL-10-dependent mechanism. We also observe that the frequency of CD19(+)CD24(hi)CD38(hi) B cell is positively correlated with the frequency of CD4(+)CD25(+)Foxp3(+)Tregs in peripheral blood. Moreover, consistent with CD19(+)CD24(hi)CD38(hi) B cell's anti-inflammatory role, we find that pneumonia patients who later developed ALI have reduced level of CD19(+)CD24(hi)CD38(hi) B cells. Together, our results demonstrated that CD19(+)CD24(hi)CD38(hi) B cells in pneumonia patients possess regulatory function in vivo, and are associated with a reduced ALI risk.