The dynamics of neuronal activation during food anticipation and feeding in the brain of food-entrained rats
The dynamics of neuronal activation during food anticipation and feeding in the brain of food-entrained rats
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DOI：
10.1016/j.brainres.2008.06.039
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发表时间：
2008-08-28
期刊：
影响因子：
2.9
通讯作者：
Tirnofeeva, Elena
中科院分区：
文献类型：
--
作者：
Poulin, Anne-Marie;Tirnofeeva, Elena
当大鼠维持在限制性喂养时间表（RFS）时，它们可以预期每天的进餐时间。食物可消化振荡器（FEO）的神经基质尚未完全了解。单个脑区的大量病变未能消除对日常饮食的行为预期，这表明FEO可能由分布式神经元网络表示。本研究的目的是检测神经元激活的动力学，作为一个标记物的c-fos mRNA的表达在大脑中的大鼠进行2小时的每日RFS，3，2和1小时前预期的一餐，在通常的喂养时间，喂养后1小时。我们还旨在澄清是否增加血浆皮质酮在食物预期大鼠一致的室旁核（PVH）的促肾上腺皮质激素释放因子（CRF）mRNA的表达增加。结果表明，进食前3 h的神经元激活并不局限于一个脑结构，但在海马前延部和隔海马核（AH/SHi）、丘脑室旁核前部（PVTa）和下丘脑背内侧核（DMH）中明显，因此代表了可能作为FEO的分布的隔海马-丘脑-下丘脑回路。进食后的神经元激活模式与食物预期过程中检测到的某些特定核团或亚区不同。血浆皮质酮在食物预期的增加并不伴随着CRF mRNA水平的增加，这表明CRF以外的因素参与RFS下肾上腺皮质分泌的控制。(C)2008 Elsevier B. V.保留所有权利。
Rats can anticipate a daily mealtime when they are maintained on restricted feeding schedules (RFS). Neural substrates of the food-entrainable oscillator (FEO) are not yet fully understood. The numerous lesions of a single brain region failed to abolish the behavioral anticipation of a daily meal, suggesting that the FEO may be represented by a distributed neuronal network. The present study was designed to detect the dynamics of neuronal activation, using as a marker the expression of c-fos mRNA in the brain of rats subjected to 2-hour daily RFS, 3, 2 and I h before the expected meal, at the time of the usual feeding, and 1 h after feeding. We also aimed to clarify whether the increase in plasma corticosterone in food-anticipating rats coincides with the increase in expression of corticotropin releasing factor (CRF) mRNA in the paraventricular hypothalamic nucleus (PVH). The obtained results revealed that the neuronal activation occurring 3 h before the expected meal was not confined to one brain structure, but was evident in the anterior hippocampal continuation and septohippocampal nucleus (AH/SHi), the anterior part of the paraventricular thalamic nucleus (PVTa), and the dorsomedial hypothalamic nucleus (DMH), thus representing distributed septohippocampal-thalamo-hypothalamic circuitry that may act as the FEO. The pattern of neuronal activation after feeding was different from that detected during food anticipation for some specific nucleus or subregions. The increase in plasma corticosterone during food anticipation was not accompanied by an increase in CRF mRNA levels, suggesting that factors other than CRF are involved in the control of adrenocortical secretion under RFS. (C) 2008 Elsevier B.V. All rights reserved.