Adenylate cyclase stimulating activity immunologically similar to parathyroid hormone‐related peptide can be extracted from fetal rat long bones
Adenylate cyclase stimulating activity immunologically similar to parathyroid hormone‐related peptide can be extracted from fetal rat long bones
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可从胎鼠长骨中提取与甲状旁腺激素相关肽免疫学相似的腺苷酸环化酶刺激活性
DOI：
--
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发表时间：
1991
影响因子：
6.2
通讯作者：
P. Bergmann
中科院分区：
文献类型：
--
作者：
N. Wolf;T. Pepersack;J. Corvilain;R. Karmali;P. Bergmann
我们观察到，用胎鼠长骨培养的培养基中含有与恶性甲状旁腺激素相关肽（PTHrP）免疫相关的肽，并刺激了犬肾皮质膜中环AMP的产生。由于刺激骨吸收时培养基的腺苷酸环化酶刺激活性（CSA）增加，因此怀疑这些肽储存在基质中并在骨吸收过程中释放。在这项工作中，我们提取了胎鼠长骨的非胶原蛋白，发现提取物中含有大量的CSA。胰蛋白酶消化后，经过滤高效液相色谱（HPLC）以24和37 kD洗脱，其生物活性消失。3-34和7-34甲状旁腺激素类似物均可抑制骨提取物的CSA和两个HPLC峰。针对甲状旁腺激素N端区域的抗血清不能阻断它，但在针对PTHrP 1-11片段的抗血清预孵育过夜后，它被显著抑制。骨提取物经SDS - PAGE电泳和抗PTHrP抗血清免疫印迹后，可观察到一条在18kd下迁移的条带。我们得出结论，在胚胎大鼠长骨基质中存在与PTHrP免疫相似的腺苷酸环化酶刺激物。在骨条件培养基中发现的低分子量腺苷酸环化酶刺激肽可能是在吸收过程中蛋白质水解形成的活性片段。
We observed that culture medium conditioned with fetal rat long bones contained peptides immunologically related to the parathyroid hormone‐related peptide of malignancy (PTHrP) and stimulated cyclic AMP production in canine renal cortical membranes. Because the adenylate cyclase stimulating activity (CSA) of the medium increased when bone resorption was stimulated, it was suspected that these peptides were stored in the matrix and released during the resorption process. In this work, we extracted the noncollagenous proteins of fetal rat long bones and found that the extract contained significant amounts of CSA. The biologic activity of the extract was abolished after trypsin digestion and eluted at 24 and 37 kD on filtration HPLC. The CSA of bone extract and of both HPLC peaks could be inhibited by 3–34 and 7–34 parathyroid hormone analogs. It was not blocked by an antiserum directed against the N‐terminal region of parathyroid hormone, but it was significantly inhibited after an overnight preincubation with an antiserum directed against the 1–11 fragment of PTHrP. One band migrating at 18 kD could be visualized after SDS‐PAGE electrophoresis of bone extract and immunoblotting with the anti‐PTHrP antiserum. We conclude that an adenylate cyclase stimulator immunologically similar to PTHrP is present in the matrix of fetal rat long bones. Adenylate cyclase stimulating peptides of lower molecular weight found in bone‐conditioned medium could be active fragments formed by proteolysis during the resorption process.
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DOI：
10.1073/pnas.87.1.108
发表时间：
1990-01-01
影响因子：
11.1
作者：
WEIR, EC;BRINES, ML;ROBBINS, RJ
通讯作者：
ROBBINS, RJ
内源性生物活性人甲状旁腺激素：通过鸟苷酸扩增肾腺苷酸环化酶测定进行测量。
DOI：
10.1210/jcem-52-5-840
发表时间：
1981
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Nissenson,RA;Abbott,SR;Teitelbaum,AP;Clark,OH;Arnaud,CD
通讯作者：
Arnaud,CD
人甲状旁腺激素（PTH）相关蛋白和人PTH：对人骨细胞和骨吸收的生物活性比较。
影响因子：
4.8
作者：
Fukayama,S;Bosma,TJ;Goad,DL;Voelkel,EF;TashjianJr,AH
通讯作者：
TashjianJr,AH
甲状旁腺激素相关肽（PTHrp）连续和短暂治疗对骨胶原合成的不同影响。
影响因子：
4.8
作者：
Canalis,E;McCarthy,TL;Centrella,M
通讯作者：
Centrella,M
比较合成人甲状旁腺激素（PTH）-（1-34）恶性肿瘤相关肽和牛PTH-（1-34）对器官培养中骨形成和吸收的影响。
影响因子：
4.8
作者：
Klein-Nulend,J;Fall,PM;Raisz,LG
通讯作者：
Raisz,LG