MR angiographic investigation of transient focal cerebral ischemia in rat
MR angiographic investigation of transient focal cerebral ischemia in rat
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DOI：
10.1002/nbm.705
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发表时间：
2001-08-01
影响因子：
2.9
通讯作者：
Hoehn, M
中科院分区：
文献类型：
--
作者：
Besselmann, M;Liu, M;Hoehn, M
将无造影剂飞行时间磁共振血管造影（TOF-MRA）应用于大鼠实验性脑卒中管腔内线闭塞模型。它与灌注加权成像和弥散加权成像（PWI 和 DWI）序列相结合，将大脑中动脉的闭塞和重新开放与这些成熟的磁共振序列的变化相关联。由于 TOF-MRA 可以不受限制地重复，因此在长达 8 小时的实验期间（2 小时对照、1 小时缺血、3-6 小时再灌注）证明了血管通畅的时间过程。 TOF-MRA 的采集时间为 10 分钟，被证明适合在纵向研究中重复分析闭塞和再灌注的血管状态。空间分辨率足以观察神经血管结构细节。在十分之八的动物中，血管造影中同侧大脑中动脉（MCA）的信号完全消失可以验证管腔内线完全闭塞血管。这与 PWI 检测到的 MCA 血管区域灌注不足（相对于对侧减少至 30.4 +/- 7.4%）以及 DWI 监测到的该区域的水离子稳态紊乱一致。一只动物在 MCA 闭塞 30 分钟后表现出延迟闭塞，另一只动物的血管闭塞失败。在 ei-ht 成功的闭塞实验中，有 7 个在撤回线后立即出现再灌注。一只动物在缝线回缩后表现出延迟再灌注。十分之三的动物可以识别出大脑中动脉血管分支的显着半球差异。总之，TOF-MRA 被认为是一种有用的方法，即使在小型实验动物中，也可以测量实验性缺血中血管闭塞和重新开放的正确时间过程，并为 PWI 监测的组织灌注状态和 DWI 检测的缺血病变区域提供补充信息。版权所有 (C) 2001 John Wiley & Sons, Ltd.
Contrast agent free time-of-flight magnetic resonance angiography (TOF-MRA) was applied to the intraluminal thread occlusion model of experimental stroke in rat. It was combined with perfusion- and diffusion-weighted imaging (PWI and DWI) sequences to correlate occlusion and reopening of the middle cerebral artery with alterations in these well-established magnetic resonance sequences. Since TOF-MRA can be repeated without limitations, the time course of vascular patency is demonstrated during an experimental period of up to 8 h (2 h control, 1 h ischemia, 3-6 h reperfusion). With an acquisition time of 10 min, TOF-MRA proved to be suitable to analyze the vascular state of occlusion and reperfusion repetitively in longitudinal studies. Spatial resolution was sufficient to observe neurovascular structural details. In eight out of 10 animals complete vessel occlusion by the intraluminal thread could be validated by an entirely extinguished signal of the ipsilateral middle cerebral artery (MCA) in the angiograms. This was in accordance with a perfusion deficit in the MCA vascular territory detected by PWI (reduction to 30.4 +/- 7.4% relative to contralateral side) and a disturbance of water ion homeostasis monitored by DWI in this area. One animal showed a delayed occlusion after 30 min of MCA occlusion, in another animal vessel occlusion failed. In seven out of the ei-ht successful occlusion experiments there was immediate reperfusion after withdrawal of the thread. One animal showed a delayed reperfusion after suture retraction. Remarkable hemispheric differences in vascular branching of the MCA could be recognized in three out of 10 animals. In conclusion, TOF-MRA is considered a helpful method to survey even in small laboratory animals the correct time course of vascular occlusion and reopening in experimental ischemia, and provides complementary information to the tissue perfusion status monitored by PWI and the ischemic lesion territory detected by DWI. Copyright (C) 2001 John Wiley & Sons, Ltd.