A genomic screen for yeast mutants defective in mitophagy.
A genomic screen for yeast mutants defective in mitophagy.
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DOI：
10.4161/auto.6.2.10901
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发表时间：
2010-02
期刊：
影响因子：
13.3
通讯作者：
Klionsky DJ
中科院分区：
文献类型：
--
作者：
Kanki T;Wang K;Klionsky DJ
线粒体自噬（mitophagy）是线粒体选择性降解的过程，在线粒体质量控制中具有重要作用。为了深入了解线粒体自噬的分子机制，我们筛选了酵母敲除文库中存在线粒体自噬缺陷的菌株。我们发现 32 个菌株表现出完全或部分线粒体自噬阻断。所鉴定的基因之一 YLR356W 是线粒体自噬所必需的，但不是巨自噬或其他类型的选择性自噬所必需的。 YLR356W 的缺失部分抑制饥饿期间的线粒体自噬，而在对数后期几乎完全抑制。因此，我们假设当细胞达到对数后阶段时，需要 Ylr356w 来检测或呈现老化或功能失调的线粒体。
Mitochondria autophagy (mitophagy) is the process of selective degradation of mitochondria that has an important role in mitochondrial quality control. To gain insight into the molecular mechanism of mitophagy, we screened a yeast knockout library for strains that are defective in mitophagy. We found 32 strains that showed a complete or partial block of mitophagy. One of the genes identified, YLR356W, is required for mitophagy, but not for macroautophagy or other types of selective autophagy. The deletion of YLR356W partially inhibits mitophagy during starvation, whereas there is almost complete inhibition at post-log phase. Accordingly, we hypothesize that Ylr356w is required to detect or present aged or dysfunctional mitochondria when cells reach the post-log phase.