Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis
Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis
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DOI：
10.1016/s1673-8527(08)60016-8
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发表时间：
2008-02-01
影响因子：
5.9
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Guo, Jingkang;Wu, Jian;Wang, Jian
热休克转录因子是调节热应激反应的主要热休克因子。它们参与调节热休克蛋白（HSPs）的表达，热休克蛋白在保护免受胁迫损伤和许多其他重要的生物学过程中至关重要。HSF基因家族的研究对于理解植物对逆境胁迫的反应机制具有重要意义。水稻（Oryza sativa）和拟南芥（Arabidopsis thaliana）的全基因组序列构成了用于比较基因组分析的宝贵资源，因为它们代表了被子植物中的两个主要进化谱系：单子叶植物和双子叶植物。在这些物种中HSF蛋白之间的系统发育关系的鉴定是一个基本的步骤，以解开新的，但尚未表征的基因属于这个家庭的功能。在本研究中，HSF基因在水稻和拟南芥中的完整互补可能已通过全基因组扫描确定。系统发育分析导致在鉴定的三个主要集群的orthopathic基因，包含属于这两个物种的成员，这必须在他们的共同祖先的被子植物的分类分裂之前表示。系统发育树的进一步分析揭示了一个可能的双子叶植物特异基因组。我们还鉴定了9对旁系同源物，为研究水稻HSF家族的进化历史和水稻基因组进化提供了证据。表达数据分析表明，HSF蛋白在植物中广泛表达。这些结果为进一步研究水稻和拟南芥HSF基因家族的功能基因组学奠定了基础。
The heat shock transcription factors (HSFs) are the major heat shock factors regulating the heat stress response. They participate in regulating the expression of heat shock proteins (HSPs), which are critical in the protection against stress damage and many other important biological processes. Study of the HSF gene family is important for understanding the mechanism by which plants respond to stress. The completed genome sequences of rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana) constitute a valuable resource for comparative genomic analysis, as they are representatives of the two major evolutionary lineages within the angiosperms: the monocotyledons and the dicotyledons. The identification of phylogenetic relationships among HSF proteins in these species is a fundamental step to unravel the functionality of new and yet uncharacterized genes belonging to this family. In this study, the full complement of HSF genes in rice and Arabidopsis has probably been identified through the genome-wide scan. Phylogenetic analyses resulted in the identification of three major clusters of orthologous genes that contain members belonging to both species, which must have been represented in their common ancestor before the taxonomic splitting of the angiosperms. Further analysis of the phylogenetic tree reveals a possible dicot specific gene group. We also identified nine pairs of paralogs, as evidence for studies on the evolution history of rice HSF family and rice genome evolution. Expression data analysis indicates that HSF proteins are widely expressed in plants. These results provide a solid base for future functional genomic studies of the HSF gene family in rice and Arabidopsis.