Assay of the multiple energy-producing pathways of mammalian cells.
Assay of the multiple energy-producing pathways of mammalian cells.
复制标题
哺乳动物细胞的多种能量产生途径的测定。
DOI：
10.1371/journal.pone.0018147
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发表时间：
2011-03-24
期刊：
影响因子：
3.7
通讯作者：
Wagner BK
中科院分区：
文献类型：
--
作者：
Bochner BR;Siri M;Huang RH;Noble S;Lei XH;Clemons PA;Wagner BK
To elucidate metabolic changes that occur in diabetes, obesity, and cancer, it is important to understand cellular energy metabolism pathways and their alterations in various cells. Here we describe a technology for simultaneous assessment of cellular energy metabolism pathways. The technology employs a redox dye chemistry specifically coupled to catabolic energy-producing pathways. Using this colorimetric assay, we show that human cancer cell lines from different organ tissues produce distinct profiles of metabolic activity. Further, we show that murine white and brown adipocyte cell lines produce profiles that are distinct from each other as well as from precursor cells undergoing differentiation. This technology can be employed as a fundamental tool in genotype-phenotype studies to determine changes in cells from shared lineages due to differentiation or mutation.
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DOI：
10.1016/s1387-2656(05)11004-7
发表时间：
2005-01-01
期刊：
BIOTECHNOLOGY ANNUAL REVIEW, VOL 11
影响因子：
--
作者：
Berridge, MV;Herst, PM;Tan, AS
通讯作者：
Tan, AS
DOI：
10.1073/pnas.0401516101
发表时间：
2004-05-11
影响因子：
11.1
作者：
Iizuka, K;Bruick, RK;Uyeda, K
通讯作者：
Uyeda, K
影响因子：
3.9
作者：
Bernas, T;Dobrucki, JW
通讯作者：
Dobrucki, JW
影响因子：
5.8
作者：
Caraccio, N;Natali, A;Ferrannini, E
通讯作者：
Ferrannini, E
影响因子：
3
作者：
FITZPATRICK, L;JENKINS, HA;BUTLER, M
通讯作者：
BUTLER, M