Identification and expression analysis of an atypical chemokine receptor-2 (ACKR2)/CC chemokine binding protein-2 (CCBP2) in rainbow trout (Oncorhynchus mykiss).
Identification and expression analysis of an atypical chemokine receptor-2 (ACKR2)/CC chemokine binding protein-2 (CCBP2) in rainbow trout (Oncorhynchus mykiss).
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DOI：
10.1016/j.fsi.2015.02.038
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发表时间：
2015-06
影响因子：
4.7
通讯作者：
Z. Qi;Yousheng Jiang;J. Holland;P. Nie;C. Secombes;Tiehui Wang
中科院分区：
文献类型：
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作者：
Z. Qi;Yousheng Jiang;J. Holland;P. Nie;C. Secombes;Tiehui Wang
非典型趋化因子受体(ACKRs)是趋化因子系统的重要组成部分，在先天免疫、获得性免疫反应和炎症反应中具有重要的调节功能。在哺乳动物中，ACKR2是炎性CC趋化因子的‘清除’受体，在活体炎症反应中发挥核心作用。在本研究中，我们已经鉴定并克隆了一种类似ACKR2的基因，这使得在另一类硬骨鱼(Holostei)、叶鳍鱼(Sarcopterygii-coelacanth)和爬行动物中进一步鉴定该分子成为可能。这些ACKR2分子的同源性得到了它们的保守结构以及系统发育树和同线分析的支持。鲑鱼ACKR2在脾和头肾中高度表达，提示该受体在限制其潜在配体的可获得性方面具有动态平衡作用。鲑鱼ACKR2的表达可在细菌和寄生虫感染的体内以及在PAMPs(聚I：C和肽聚糖)和细胞因子(IL-6、肿瘤坏死因子-α、干扰素-γ和IL-21)的作用下以时间依赖的方式调节。这些表达和调节模式表明，鲑鱼ACKR2以一种复杂的方式受到调控，并在鱼类和哺乳动物中的趋化因子网络控制中发挥重要作用。
Atypical chemokine receptors (ACKRs) have emerged as key components of the chemokine system, with an essential regulatory function in innate and adaptive immune responses and inflammation. In mammals ACKR2 is a ‘scavenging’ receptor for inflammatory CC chemokines and plays a central role in the resolution ofin vivoinflammatory responses. An ACKR2 like gene has been identified and cloned in rainbow trout (Teleostei) in the present study, enabling the further identification of this molecule in another group of ray-finned teleost fish (Holostei), in a lobe-finned fish (Sarcopterygii-coelacanth), and in reptiles. The identity of these ACKR2 molecules is supported by their conserved structure, and by phylogenetic tree and synteny analysis. Trout ACKR2 is highly expressed in spleen and head kidney, suggesting a homeostatic role of this receptor in limiting the availability of its potential ligands. Trout ACKR2 expression can be modulatedin vivoby bacterial and parasitic infections, andin vitroby PAMPs (poly I:C and peptidoglycan) and cytokines (IL-6, TNF-α, IFN-γ and IL-21) in a time dependent manner. These patterns of expression and modulation suggest that trout ACKR2 is regulated in a complex way and has an important role in control of the chemokine network in fish as in mammals.