ORGANIZATION AND EVOLUTION OF AN ALPHA-SATELLITE DNA SUBSET SHARED BY HUMAN CHROMOSOME-13 AND CHROMOSOME-21
ORGANIZATION AND EVOLUTION OF AN ALPHA-SATELLITE DNA SUBSET SHARED BY HUMAN CHROMOSOME-13 AND CHROMOSOME-21
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DOI：
10.1007/bf00160427
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发表时间：
1993-11-01
影响因子：
3.9
通讯作者：
WILLARD, HF
中科院分区：
文献类型：
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作者：
GREIG, GM;WARBURTON, PE;WILLARD, HF
来自人类 13 号和 21 号染色体（D13Z1 和 D21Z1）共享子集的 α 卫星 DNA 高阶重复 (HOR) 单元的结构已被详细检查。通过使用一组拥有 13 号染色体或 21 号染色体的杂种，可以区分 13 号和 21 号染色体上的不同 HOR 单位基因型。我们还确定了在约 8% 的 13 号染色体上发现的变异 HOR 单元结构的基础，但在 21 号染色体上根本没有发现。构建并比较了在两个 13 号染色体基因型和 21 号染色体基因型上发现的 HOR 单元的基因组限制图谱。来自 D13Z1/D21Z1 子集的主要 1.9 千碱基对 HOR 单元的核苷酸序列已确定。对构成 HOR 的不同 α 卫星单体的 DNA 序列进行比较，并使用这些数据开发一个基于不等交换的模型，用于在这两条染色体的着丝粒处发现的当前 HOR 单元的进化。
The structure of the alpha satellite DNA higher-order repeat (HOR) unit from a subset shared by human chromosomes 13 and 21 (D13Z1 and D21Z1) has been examined in detail. By using a panel of hybrids possessing either a chromosome 13 or a chromosome 21, different HOR unit genotypes on chromosomes 13 and 21 have been distinguished. We have also determined the basis for a variant HOR unit structure found on approximately 8% of chromosomes 13 but not at all on chromosomes 21. Genomic restriction maps of the HOR units found on the two chromosome 13 genotypes and on the chromosome 21 genotype are constructed and compared. The nucleotide sequence of a predominant 1.9-kilobasepair HOR unit from the D13Z1/D21Z1 subset has been determined. The DNA sequences of different alpha satellite monomers comprising the HOR are compared, and the data are used to develop a model, based on unequal crossing-over, for the evolution of the current HOR unit found at the centromeres of both these chromosomes.