Localized expression of genes related to carbohydrate and lipid absorption along the crypt-villus axis of rat jejunum
Localized expression of genes related to carbohydrate and lipid absorption along the crypt-villus axis of rat jejunum
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DOI：
10.1016/j.bbagen.2009.08.004
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发表时间：
2009-12-01
影响因子：
3
通讯作者：
Goda, Toshinao
中科院分区：
文献类型：
--
作者：
Suzuki, Takuji;Mochizuki, Kazuki;Goda, Toshinao
背景资料：空肠的肠上皮细胞在这些细胞从隐窝快速分化为绒毛细胞时表达与营养物和离子的消化/吸收相关的几个基因。然而，目前还不清楚是否广泛的基因表达的分布沿着绒毛-隐窝轴的空肠在分化过程中发生了变化。方法：我们研究了空肠基因表达的变化，在大鼠从隐窝细胞分化为绒毛细胞时，使用DNA微阵列分析绒毛-隐窝柱的低温切片。结果：在分化过程中，许多与细胞生长相关的基因的表达迅速下降，而与营养物质和离子的消化和吸收相关的基因的表达增加。与淀粉和蔗糖的消化和吸收相关的基因的子集的表达在绒毛的中部最高，而与膳食脂肪吸收相关的基因的表达在绒毛的顶部最高。几个转录因子，如Pdx 1，Foxa 2和Thra的表达在隐窝，而Klf 15的高表达在隐窝绒毛过渡。Klf 4和Pparg在绒毛顶部表达最高。结论：与淀粉/蔗糖和膳食脂肪的消化吸收相关的基因及其转录因子/辅因子在隐窝-绒毛轴沿着的特定位置表达。一般意义：空肠可以通过沿绒毛-隐窝轴沿着同时表达基因子集来有效地吸收营养。(C)2009爱思唯尔有限公司版权所有。
Background: Enterocytes of the jejunum express several genes related to digestion/absorption of nutrients and ions when these cells rapidly differentiate from crypt to villus cells. However, it is unknown whether the distribution of extensive gene expression along the villus-crypt axis of the jejunum is altered during differentiation.Methods: We investigated the changes in jejunal gene expression during differentiation from crypt to villus cells in rats using DNA microarray analysis on cryorstat sections of the villus-crypt columns.Results: During differentiation, the expression of many genes related to cell growth rapidly decreased, while expression of genes related to digestion and absorption of nutrients and ions increased. Expression of a subset of genes related to the digestion and absorption of starch and sucrose was highest at the middle of the villi, whereas expression of genes related to dietary fat absorption was highest at the top of the villi. Several transcriptional factors such as Pdx1, Foxa2 and Thra were expressed in the crypt, whereas Klf15 was highly expressed during the crypt-villus transition. Expression of Klf4 and Pparg was highest at the top of the villi.Conclusions: Subsets of genes related to the digestion and absorption of starch/sucrose and dietary fat as well as their transcriptional factors/co-factors are expressed in the specific locations along the crypt-villus axis. General Significance: The jejunum may absorb nutrients effectively by simultaneously expressing subsets of genes along the villus-crypt axis. (C) 2009 Elsevier B.V. All rights reserved.