A comprehensive characterization of the peptide and protein constituents of human seminal fluid
A comprehensive characterization of the peptide and protein constituents of human seminal fluid
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DOI：
10.1002/pros.20089
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发表时间：
2004-10-01
期刊：
影响因子：
2.8
通讯作者：
Duncan, MW
中科院分区：
文献类型：
--
作者：
Fung, KYC;Glode, LM;Duncan, MW
背景。对于精液中的肽和蛋白质成分及其在前列腺疾病（包括良性前列腺增生和前列腺癌）中的作用的了解还很少。我们对精液进行了蛋白质组分析，以此作为识别前列腺疾病敏感和特异性诊断标记物的先行者；帮助改善治疗干预；并且，增强我们对前列腺健康和疾病的了解。方法。通过凝胶电泳（1D 和 2D）分离汇集的人精液 (n = 5) 的肽和蛋白质成分，并通过基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF-MS) 或毛细管液相色谱串联质谱 (LC-MS/MS) 进行鉴定。 结果。二维电泳 (2DE) 分析表明，大多数蛋白质存在多种翻译后变体。检测并鉴定了激素、生长因子和生物活性肽。结论。我们已鉴定出正常人精液中的 100 多种蛋白质和肽成分。 (C) 2004 Wiley-Liss, Inc.
BACKGROUND. Knowledge of the peptide and protein components of seminal fluid and their role in prostate diseases including benign prostatic hyperplasia and prostate carcinoma is scant. We have undertaken a proteomic analysis of semen as a forerunner to identifying sensitive and specific diagnostic markers of prostatic diseases; to aid in improved therapeutic intervention; and, to enhance our understanding of prostate health and disease.METHODS. Peptide and protein components of pooled human seminal fluid (n = 5) were separated by gel electrophoresis (1D and 2D) and identified by either matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) or capillary liquid chromatography tandem mass spectrometry (LC-MS/MS).RESULTS. Analysis by two-dimensional electrophoresis (2DE) established that there were multiple post-translational variants of the majority of the proteins. Hormones, growth factors and bioactive peptides were detected and identified.CONCLUSIONS. We have identified over 100 protein and peptide components of normal human seminal fluid. (C) 2004 Wiley-Liss, Inc.