Determination of cell/medium interfacial tensions from contact angles in aqueous polymer systems.
Determination of cell/medium interfacial tensions from contact angles in aqueous polymer systems.
复制标题
根据水性聚合物体系中的接触角测定细胞/介质界面张力。
DOI：
10.1016/0005-2736(81)90480-6
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
D. McIver
中科院分区：
文献类型：
--
作者：
S. Schürch;D. Gerson;D. McIver
利用葡聚糖和聚乙二醇双水相体系中的一系列聚合物液滴在细胞层上的接触角来确定扩散到细胞层的临界界面张力或极限界面张力。较致密的富葡聚糖相的测试液滴是在较轻的聚乙二醇富相中形成的。用悬滴法测定了两相间的界面张力γ，发现γ−1~2与液滴与细胞层夹角的余弦呈线性关系(Good-Girivalco图)。因此，我们能够确定在细胞层上扩散的极限或临界界面张力γc。γc的值是电池/沐浴介质界面的界面能的量度。测得人红细胞和中性粒细胞的γc分别为0.6 5和0.84μN·m−1，猪肺巨噬细胞为0.93μN·m−1，各种转化的小鼠淋巴细胞系为0.75~3.6 0μN·m−1，小鼠脾淋巴细胞为2.5 3μN·m−1。不同药物对小鼠脾淋巴细胞γc的影响不同，刀豆蛋白A可降低小鼠脾淋巴细胞γc，细菌脂多糖可使小鼠脾淋巴细胞γc升高。钙离子载体A23187可增加猪肺巨噬细胞和小鼠脾淋巴细胞的γc。这种新的方法提供了一种定量的方法来研究细胞表面的能量和疏水性，它们被认为在膜介导的现象和细胞黏附中起着重要的作用。
The contact angles on cell layers of a series of polymeric droplets from aqueous two-phase systems of dextran and poly (ethylene glycol) have been used to determine the critical or limiting interfacial tension for spreading on the cell layers. Test droplets of the denser dextran-rich phase were formed in the lighter poly (ethylene glycol)-rich phase. The interfacial tensions, γ, between the phases were determined with the pendant drop method, and a linear relationship was found between γ− 1 2 and the cosine of the angle the droplets made with the cell layers (Good-Girifalco plot). We were thus able to determine the limiting or critical interfacial tension, γ c, for spreading on the cell layers. The value of γ c is a measure of the interfacial energy of the cell/bathing medium interface. Values of γ c obtained by this method include the following: 0.65 and 0.84 μN· m− 1 for human erythrocytes and neutrophils, respectively; 0.93 μN· m− 1 for porcine pulmonary macrophages; 0.75–3.60 μN· m− 1 for various transformed murine lymphoid cell lines, and 2.53 μN· m− 1 for Balb/c murine spleen lymphocytes. Exposure to various agents has differing effects on γ c. Concanavalin A reduces γ c, and bacterial lipopolysaccharide increases γ c of murine spleen lymphocytes. The calcium ionophore, A23187, increases γ c of both porcine pulmonary macrophages and murine spleen lymphocytes. This new method provides a quantitative approach to the cell surface energy and hydrophobicity which are thought to play an important role in membrane-mediated phenomena and in cell adhesion.