Exploring the Genomic Diversity and Cariogenic Differences of Streptococcus mutans Strains Through Pan-Genome and Comparative Genome Analysis
Exploring the Genomic Diversity and Cariogenic Differences of Streptococcus mutans Strains Through Pan-Genome and Comparative Genome Analysis
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DOI：
10.1007/s00284-017-1305-z
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发表时间：
2017-10-01
影响因子：
2.6
通讯作者：
Chen, Feng
中科院分区：
文献类型：
--
作者：
Meng, Peiqi;Lu, Chang;Chen, Feng
泛基因组是指可以在给定细菌物种中发现的基因的总和，包括核心基因组和核心基因组。本研究对183株变形链球菌（Streptococcus mutans，S.从泛基因组的角度分析了变异株）分离株。分析表明，S.变异人有一个“开放的”泛基因组，这意味着随着更多的基因组被测序，有大量的新基因有待发现。此外，S.变形杆菌具有有限的核心基因组，其由与细菌内的生命活动相关的基因组成，例如代谢和遗传信息存储或处理，分别占核心基因的35.6%和26.6%。我们估计理论上的核心基因组大小约为1083个基因，这比其他链球菌物种少。此外，与那些分布不太广泛的基因相比，核心基因遭受更大的选择压力。不出意料，S.变异株与龋齿状态无关，表明其他因素也是龋齿发生的原因。这些结果有助于更好地了解物种的进化特征和基因组组成的动态变化。这也有助于形成预防龋齿的新理论基础。此外，该研究为从泛基因组角度分析大型病原体基因组数据集提供了一个范例。
Pan-genome refers to the sum of genes that can be found in a given bacterial species, including the core-genome and the dispensable genome. In this study, the genomes from 183 Streptococcus mutans (S. mutans) isolates were analyzed from the pan-genome perspective. This analysis revealed that S. mutans has an "open" pan-genome, implying that there are plenty of new genes to be found as more genomes are sequenced. Additionally, S. mutans has a limited core-genome, which is composed of genes related to vital activities within the bacterium, such as metabolism and hereditary information storage or processing, occupying 35.6 and 26.6% of the core genes, respectively. We estimate the theoretical core-genome size to be about 1083 genes, which are fewer than other Streptococcus species. In addition, core genes suffer larger selection pressures in comparison to those that are less widely distributed. Not surprisingly, the distribution of putative virulence genes in S. mutans strains does not correlate with caries status, indicating that other factors are also responsible for cariogenesis. These results contribute to a more understanding of the evolutionary characteristics and dynamic changes within the genome components of the species. This also helps to form a new theoretical foundation for preventing dental caries. Furthermore, this study sets an example for analyzing large genomic datasets of pathogens from the pan-genome perspective.