Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry
Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry
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DOI：
10.1039/b716115h
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发表时间：
2008-01-01
影响因子：
4.9
通讯作者：
Tate, Edward W.
中科院分区：
文献类型：
--
作者：
Heal, William P.;Wickramasinghe, Sasala R.;Tate, Edward W.
n -肉豆荚酰基转移酶介导的含叠氮化物底物修饰是一种高选择性和实用的方法，用于体外和体内重组蛋白的n端标记。
N-Myristoyl transferase- mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.