AUTOCRINE INDUCTION OF COLLAGENASE BY SERUM AMYLOID A-LIKE AND BETA-2-MICROGLOBULIN LIKE PROTEINS
AUTOCRINE INDUCTION OF COLLAGENASE BY SERUM AMYLOID A-LIKE AND BETA-2-MICROGLOBULIN LIKE PROTEINS
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DOI：
10.1126/science.2536953
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发表时间：
1989-02-03
期刊：
影响因子：
56.9
通讯作者：
BENSON, MD
中科院分区：
文献类型：
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作者：
BRINCKERHOFF, CE;MITCHELL, TI;BENSON, MD
鉴定了两种诱导兔成纤维细胞胶原酶合成的自分泌蛋白（14和21千道尔顿）。这些蛋白是从兔滑膜成纤维细胞经肉豆蔻酸佛波刺激的无血清培养基中纯化出来的。14千道尔顿和12千道尔顿种的氨基末端序列为。60%到80%与血清淀粉样蛋白A和。β同源。2个微球蛋白。聚丙烯酰胺凝胶洗脱的蛋白保留了诱导兔和人成纤维细胞合成胶原酶的能力。这些自分泌蛋白可能提供了一种在正常重塑以及炎症和疾病状态下调节胶原酶合成的手段。
Two autocrine proteins of 14 and 21 kilodaltons that induce the synthesis of rabbit fibroblast collagenase were identified. The proteins were purified from serum-free culture medium taken from rabbit synovial fibroblasts stimulated with phorbol myristate acetate. The amino-terminal sequences of the 14- and 12-kilodalton species were .apprx. 60 to 80 percent homologous with serum amyloid A and .beta.2 microglobulin, respectively. The polyacrylamide gel-eluted proteins retained the ability to induce collagenase synthesis in rabbit and human fibroblasts. These autocrine proteins may provide a means to modulate collagenase synthesis in normal remodeling as well as in inflammation and disease states.