Structure of the FBJ murine osteosarcoma virus genome: molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cells
Structure of the FBJ murine osteosarcoma virus genome: molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cells
复制标题
FBJ 鼠骨肉瘤病毒基因组的结构：其相关辅助病毒的分子克隆以及来自小鼠和人类细胞的 v-fos 基因的细胞同源物
DOI：
10.1128/mcb.3.5.914-921.1983
复制
发表时间：
1983
影响因子：
5.3
通讯作者：
Inder M. Verma
中科院分区：
文献类型：
--
作者：
Tom Curran;William P. MAcCONNELL;Flip;Van;Straaten;Inder M. Verma
FBJ 鼠白血病病毒 (FBJ-MLV) 的 8.2-kilobase (kb) 未整合环状 DNA 形式通过在单个 HindIII 位点切割而线性化，分子克隆到噬菌体 Charon 30 中，随后亚克隆到 pBR322 (pFBJ-MLV-1) 中。 FBJ-MLV 病毒体 RNA 和 pFBJ-MLV-1 DNA 均用于通过与克隆的 FBJ-MSV 前病毒 DNA 形成异源双链体来研究辅助病毒序列在 FBJ 鼠骨肉瘤病毒基因组 (FBJ-MSV) 中的排列。结果显示，FBJ-MSV基因组在其5'末端含有0.8 kb的辅助病毒序列，在其3'末端含有0.98 kb的辅助病毒序列。大约 6.8 kb 的辅助病毒序列已被删除，并且 1.7 kb 的无关序列被插入到 FBJ-MSV 基因组中。该取代区域包含 v-fos，即 FBJ-MSV 的转化基因。使用 v-fos 特异性探针，我们从小鼠和人类染色体 DNA 中克隆了同源序列 (c-fos)。对这些重组克隆的 FBJ-MSV DNA 进行异源双链分析表明，c-fos（小鼠）和 c-fos（人）序列均与整个 1.7 kb v-fos 区域杂交。然而，对于FBJ-MSV基因组，从5'至3'，0.27、0.26、0.14、0.5和0.5kb的五个同源区域被0.76、0.55、0.1和0.1kb的四个非同源区域分开。这些序列的大小在 c-fos（小鼠）和 c-fos（人类）中显示出惊人的相似性。
The 8.2-kilobase (kb) unintegrated circular DNA form of the FBJ murine leukemia virus (FBJ-MLV) was linearized by cleavage at the single HindIII site, molecularly cloned into bacteriophage Charon 30, and subsequently subcloned into pBR322 (pFBJ-MLV-1). Both FBJ-MLV virion RNA and pFBJ-MLV-1 DNA were used to investigate the arrangement of helper virus sequences in the FBJ murine osteosarcoma virus genome (FBJ-MSV) by heteroduplex formation with cloned FBJ-MSV proviral DNA. The results showed that the FBJ-MSV genome contained 0.8 kb of helper virus sequence at its 5' terminus and 0.98 kb at its 3' terminus. Approximately 6.8 kb of helper virus sequence had been deleted, and 1.7 kb of unrelated sequence was inserted into the FBJ-MSV genome. This substituted region contains v-fos, the transforming gene of FBJ-MSV. Using a probe specific for v-fos, we have cloned homologous sequences (c-fos) from mouse and human chromosomal DNA. Heteroduplex analysis of FBJ-MSV DNA with these recombinant clones showed that both the c-fos(mouse) and the c-fos(human) sequences hybridized to the entire 1.7-kb v-fos region. However, five regions of homology of 0.27, 0.26, 0.14, 0.5, and 0.5 kb were separated by four regions of nonhomology of 0.76, 0.55, 0.1, and 0.1 kb from 5' to 3' with respect to the FBJ-MSV genome. The size of these sequences showed striking similarity in both c-fos(mouse) and c-fos(human).