GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION
GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION
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DOI：
10.1126/science.8303295
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发表时间：
1994-02-11
期刊：
影响因子：
56.9
通讯作者：
PRASHER, DC
中科院分区：
文献类型：
--
作者：
CHALFIE, M;TU, Y;PRASHER, DC
维多利亚水母绿色荧光蛋白（GFP）的互补DNA在原核（大肠杆菌）或真核（秀丽隐杆线虫）细胞中表达时产生荧光产物。由于这种荧光不需要外源底物和辅因子，因此GFP表达可用于监测活生物体中的基因表达和蛋白质定位。
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.