Molecular structure of agarose chains in thermoreversible hydrogels revealed by means of a fluorescent probe technique
Molecular structure of agarose chains in thermoreversible hydrogels revealed by means of a fluorescent probe technique
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DOI：
10.1002/polb.20374
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发表时间：
2005-03-15
影响因子：
--
通讯作者：
Watase, M
中科院分区：
文献类型：
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作者：
Itagaki, H;Fukiishi, H;Watase, M
用1-苯胺基-8-萘磺酸（ANS）荧光探针法研究了高分子量和低分子量琼脂糖热可逆水凝胶。ANS在琼脂糖/水溶液中的荧光光谱随琼脂糖浓度的增加而明显变化。对光谱的分析表明，这种变化不是由于整个光谱的移位，而是由于水中的游离ANS分子，除了在542 nm处的荧光峰之外，还存在ANS的两个荧光组分。假设461 nm处的荧光组分来自插入单个31螺旋形式琼脂糖链或由化学缺陷产生的非极性区域内的ANS，并且假设507和522 nm之间的荧光组分来自具有松散螺旋结构的琼脂糖链的聚集区域中的ANS。毫无疑问，一定数量的水分子被包括在聚集区域中。(C)2005 Wiley Periodicals，Inc.
Thermoreversible hydrogels of agarose with both high and low molecular weights were studied by means of a fluorescence probe method using 1-anilino-8-naphthalene sulfonic acid (ANS). The fluorescence spectra of ANS in agarose/water changed markedly with agarose concentration. Analysis of the spectra shows that this change is not due to a shift of the whole spectrum, but that there are two fluorescent components of ANS in addition to the fluorescence peak at 542 nm due to free ANS molecules in water. The fluorescence component at 461 nm is assumed to be from ANS intercalated within either a single 31 helix-form agarose chain or nonpolar region produced by chemical defect, and the fluorescence component between 507 and 522 nm is assumed to be from ANS in aggregated regions of agarose chains having a loose helical structure. There is no doubt that a certain number of water molecules are included in the aggregated region. (C) 2005 Wiley Periodicals, Inc.