Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering.
Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering.
复制标题
通过光诱导 T 跃迁和时间分辨 X 射线溶液散射揭示胰岛素六聚体解离动力学。
DOI：
10.1039/c8pp00034d
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Chen,LinX
中科院分区：
文献类型：
--
作者：
Rimmerman,Dolev;Leshchev,Denis;Hsu,DarrenJ;Hong,Jiyun;Abraham,Baxter;Kosheleva,Irina;Henning,Robert;Chen,LinX
利用光诱导温度跃变技术和时间分辨X射线散射技术研究了胰岛素六聚体解离的结构动力学。六聚体解离的过程中，发现涉及到几个短暂的中间体，包括一个扩展的六聚体和一个不稳定的四聚体。我们的研究结果提供了深入了解蛋白质-蛋白质协会的机制。
The structural dynamics of insulin hexamer dissociation were studied by the photoinduced temperature jump technique and monitored by time-resolved X-ray scattering. The process of hexamer dissociation was found to involve several transient intermediates, including an expanded hexamer and an unstable tetramer. Our findings provide insights into the mechanisms of protien–protein association.