cDNA array analysis of cytobrush-collected normal and malignant cervical epithelial cells: a feasibility study
cDNA array analysis of cytobrush-collected normal and malignant cervical epithelial cells: a feasibility study
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DOI：
10.1016/j.cancergencyto.2004.08.007
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发表时间：
2005-04-01
影响因子：
--
通讯作者：
Manavi, M
中科院分区：
文献类型：
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作者：
Hudelist, G;Czerwenka, K;Manavi, M
基因表达谱分析是评价几种人类恶性肿瘤生物学行为和临床预后的有效方法。目的探讨宫颈鳞癌细胞中差异表达基因的表达谱，以及从宫颈拭子中提取宫颈鳞癌细胞进行基因表达谱分析的可行性。用cDNA芯片分析了3例高危型人乳头瘤病毒（HR-HPV）阳性的浸润性宫颈鳞癌和3例HPV阴性的宫颈涂片正常的宫颈鳞状细胞。进行免疫印迹分析以用cDNA阵列检测对应于最高上调基因的蛋白质。ERBB 2、KIT、FLT 1、MYCN、RAS、CDKN 2A、CCND 1、NME 1、NME 2、MET、FGF 7、FGFR 2和STAT 1的mRNA表达在恶性标本中增加。一些与抗凋亡（如BCL 2），细胞结构，或细胞附着相关的表达基因也在癌细胞中上调。观察到转化生长因子受体超家族（TGF）和整合素家族成员、白细胞介素1（IL 1）和胰岛素样生长因子结合蛋白（IGFBPs）的基因表达降低。这项研究表明，通过确定一个特征性的基因表达模式，明确区分恶性和正常宫颈上皮鳞状细胞类型的宫颈鳞状细胞的细胞刷获得的基因表达谱的可行性。我们假设，这种非侵入性技术可用于评估模糊巴氏涂片（PAP）。(c)2005年爱思唯尔公司All rights reserved.
Analysis of gene expression pattern is a useful approach to evaluating the biological behavior and clinical outcome of several human malignancies. Differentially expressed genes in malignant squamous cervical cells and the feasibility of gene expression profiling on squamous cervical cells obtained from cervical swabs were investigated. Cervical squamous cells from three women with high-risk human papilloma virus (HR-HPV) positive invasive squamous cervical carcinoma and from three HPV-negative women with normal ectocervical smears were analyzed with cDNA array. Immunoblot analysis was performed to detect the proteins corresponding to the highest upregulated genes with cDNA array. mRNA expression of ERBB2, KIT, FLT1, MYCN, RAS, CDKN2A, CCND1, NME1, NME2, MET, FGF7, FGFR2, and STAT1 was increased in malignant samples. Several expressed genes associated with antiapoptosis (Such as BCL2), cell structuring, or cell attachment were also upregulated in carcinoma cells. Decreased gene expression was observed for members of the transforming growth factor receptor superfamily (TGF) and integrin family, interleukin 1 (IL1), and insulin-like growth factor binding proteins (IGFBPs). This study shows the feasibility of gene expression profiling of cervical squamous cells obtained with cytobrushes by identifying a characteristic gene expression pattern that clearly distinguishes between malignant and normal cervical epithelia of squamous type. We hypothesize that this noninvasive technique could be used in the evaluation of ambiguous Papanicolaou (PAP) smears. (c) 2005 Elsevier Inc. All rights reserved.