Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola
Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola
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DOI：
10.1111/imb.12526
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发表时间：
2018-12-01
影响因子：
2.6
通讯作者：
de la Fuente, J.
中科院分区：
文献类型：
--
作者：
Diaz-Sanchez, S.;Hernandez-Jarguin, A.;de la Fuente, J.
库蠓属蠓是已知的影响人类和动物健康的虫媒病毒载体。然而，人们对库蠓的微生物群及其对库蠓生物学的影响知之甚少。在这项研究中，生物和非生物因素对C. imicola微生物群的影响采用全身DNA样本的散弹宏基因组测序来表征。在意大利西西里岛的两个地点采集了野生捕获的无产卵期姬蠓成虫。记录了两地温度和土壤湿度的气候变量以及潜在的宿主血源。假单胞菌、埃希氏菌、Halomonas、Zinderia Candidatus、Propionibacterium和Schizosaccharomyces在C. imicola群体中共享核心微生物组。在不同的生境中还发现了独特的类群，突出了两个种群微生物组成的相似性和差异性。DNA和蛋白质鉴定表明，两个种群对宿主的偏好存在差异，智人和家犬是两个地区首选的血源。主成分分析表明，寄主偏好（智人）和当地土壤湿度因素共同影响了野生密枝螟种群的微生物组成。这些结果有助于表征微生物组在昆虫适应中的作用，并有助于预测库蠓物种的地理扩展，从而对病媒传播疾病的控制具有潜在意义。
Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect's biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota was characterized using shotgun-metagenomic sequencing of whole-body DNA samples. Wild-caught C. imicola adult nulliparous females were sampled in two locations from Sicily, Italy. The climatic variables of temperature and soil moisture from both localities were recorded together with potential host bloodmeal sources. Shared core microbiome among C. imicola populations included Pseudomonas, Escherichia, Halomonas, Candidatus Zinderia, Propionibacterium, and Schizosaccharomyces. Specific and unique taxa were also found in C. imicola from each location, highlighting similarities and differences in microbiome composition between the two populations. DNA and protein identification showed differences in host preferences between the two populations, with Homo sapiens and Canis lupus familiaris L. being the preferred bloodmeal source in both locations. A principal component analysis showed that the combined effect of host preferences (H. sapiens) and local soil moisture factors shape the microbiome composition of wild-caught populations of C. imicola. These results contribute to characterizing the role of the microbiome in insect adaptation and its utility in predicting geographic expansion of Culicoides species with potential implications for the control of vector-borne diseases.