Blood volume and cardiac index in rats after exchange transfusion with hemoglobin-based oxygen carriers.
Blood volume and cardiac index in rats after exchange transfusion with hemoglobin-based oxygen carriers.
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用基于血红蛋白的氧载体换血后大鼠的血容量和心脏指数。
DOI：
10.1152/jappl.1997.82.6.1995
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Winslow,RM
中科院分区：
文献类型：
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作者：
Migita,R;Gonzales,A;Gonzales,ML;Vandegriff,KD;Winslow,RM
米吉塔、拉塞尔、阿曼多·冈萨雷斯、玛丽亚·l·冈萨雷斯、金·d·范德格里夫和罗伯特·m·温斯洛。输血后大鼠血容量和心脏指数的变化。达成。生理学报，32(6):995 - 998。我们用人血红蛋白α-链与富马酸双（3,5-二溴水杨基）（α ααHb）和聚乙二醇修饰的牛血红蛋白（PEGHb）交联，在50%等容交换输注后，测量了大鼠血浆容量和心脏指数。ααHb和PEGHb在胶体渗透压（分别为23.4和118.0 Torr）、氧亲和力（血红蛋白氧半饱和压分别为30.0和10.2 Torr）、粘度（分别为1.00和3.39 cP）和分子量（分别为64,400和105,000）方面存在差异。血浆体积测定采用经血浆血红蛋白干扰改性的埃文斯蓝染料稀释液。与α ααHb（49.0±1.2 ml/kg）或乳酸林格液（48.0±2.0 ml/kg）或对照组（58.2±1.9 ml/kg）相比，peghb处理动物的血容量显著升高（74.0±3.5 ml/kg）。ααHb交换后的心率降低与血容量收缩的预期相反，提示ααHb可能具有直接的心肌抑制作用。PEGHb在注射后2小时内明显缓慢的消除是血浆体积膨胀的结果：当比较PEGHb和ααHb的绝对血红蛋白（浓度×血浆体积）时，发现它们的消除速度没有差异。这些研究强调，当评估无细胞血红蛋白对血流动力学测量的影响时，需要了解血容量调节。
Migita, Russell, Armando Gonzales, Maria L. Gonzales, Kim D. Vandegriff, and Robert M. Winslow.Blood volume and cardiac index in rats after exchange transfusion with hemoglobin-based oxygen carriers.J. Appl. Physiol.82(6): 1995–2002, 1997.—We have measured plasma volume and cardiac index in rats after 50% isovolemic exchange transfusion with human hemoglobin cross-linked between the α-chains with bis(3,5-dibromosalicyl)fumarate (ααHb) and with bovine hemoglobin modified with polyethylene glycol (PEGHb). ααHb and PEGHb differ in colloid osmotic pressure (23.4 and 118.0 Torr, respectively), oxygen affinity (oxygen half-saturation pressure of hemoglobin = 30.0 and 10.2 Torr, respectively), viscosity (1.00 and 3.39 cP, respectively), and molecular weight (64,400 and 105,000, respectively). Plasma volume was measured by Evans blue dye dilution modified for interference by plasma hemoglobin. Blood volumes in PEGHb-treated animals were significantly elevated (74.0 ± 3.5 ml/kg) compared with animals treated with ααHb (49.0 ± 1.2 ml/kg) or Ringer lactate (48.0 ± 2.0 ml/kg) or with controls (58.2 ± 1.9 ml/kg). Heart rate reduction after ααHb exchange is opposite to that expected with blood volume contraction, suggesting that ααHb may have a direct myocardial depressant action. The apparently slow elimination of PEGHb during the 2 h after its injection is a consequence of plasma volume expansion: when absolute hemoglobin (concentration × plasma volume) is compared for PEGHb and ααHb, no difference in their elimination rates is found. These studies emphasize the need to understand blood volume regulation when the effects of cell-free hemoglobin on hemodynamic measurements are evaluated.