Spectral imaging microscopy web sites and data
Spectral imaging microscopy web sites and data
复制标题
DOI：
10.1002/cyto.a.20304
复制
发表时间：
2006-08-01
期刊：
影响因子：
3.7
通讯作者：
Boswell, Carl
中科院分区：
文献类型：
--
作者：
McNamara, George;Gupta, Amit;Boswell, Carl
与一代人以前相比，互联网使人们能够更容易地访问光谱成像出版物、光谱图和数据。在这期《细胞计数》中讨论的光谱成像系统之所以能工作，是因为试剂和硬件光谱是可再现的、可重复使用的，并为光谱分解和光谱成分识别算法提供输入。这些光谱需要随时可用，以便确定购买什么，如何使用它，以及输出意味着什么。我们参考了几个商业赞助和学术光谱网站，并讨论了我们的光谱绘图和数据网站。网站包括来自Invitrogen/Molecular Probes、BD Biosciences、Zeiss/Bio-Rad Cell Sciences的荧光染料图服务器，以及来自Chroma Technology和Omega Optical的滤光片组服务器。其中一些网站包括数据下载功能。最近，两家显微镜制造商在他们的网站上公布了选择物镜的透射曲线-对于任何进行多光子激发显微镜的人来说都是至关重要的数据。值得注意的是，PhotoChemCAD 2.0有超过200种染料和一个可下载的数据库/绘图程序，USC-A化学紫外可见光数据库显示了临床组织学和病理学中使用的许多染料和指示剂的吸收光谱。我们的荧光光谱图形/计算器网站提供了来自其中许多来源和其他来源的染料、滤光片和照明数据。PubSpectra是我们的免费下载网站，它使用Microsoft Excel文件作为标准化的人/机可读格式，包含2，000多个生物医学光谱。数据不受版权保护的原则提供了一个框架，在这个框架内，所有科学数据都应可自由获取。(c)2006年国际分析细胞学学会。
The Internet is enabling greater access to spectral imaging publications, spectral graphs, and data than that was available a generation ago. The spectral imaging systems discussed in this issue of Cytometry work because reagent and hardware spectra are reproducible, reusable, and provide input to spectral unmixing and spectral components recognition algorithms. These spectra need to be readily available in order to determine what to purchase, how to use it, and what the output means. We refer to several commercially sponsored and academic spectral web sites and discuss our spectral graphing and data sites. Sites include fluorescent dye graph servers from Invitrogen/Molecular Probes, BD Biosciences, Zeiss/Bio-Rad Cell Sciences, and filter set servers from Chroma Technology and Omega Optical. Several of these sites include data download capabilities. Recently, two microscope manufacturers have published on their web sites transmission curves for select objective lenses-crucial data for anyone doing multiphoton excitation microscopy Notable among the academic sites, PhotoChemCAD 2.0 has over 200 dyes and a downloadable database/graphing program, and the USC-A Chemistry UV-vis Database displays absorption spectra of many dyes and indicators used in clinical histology and pathology. Our Fluorescent Spectra graphing/calculator site presents dyes, filters, and illumination data from many of these and additional sources. PubSpectra is our free download site which uses Microsoft Excel files as standardized human/machine readable format with over 2,000 biomedical spectra. The principle that data is not subject to copyright provides a framework in which all scientific data should be made freely accessible. (c) 2006 International Society for Analytical Cytology.