EFFECT OF CASTRATION AND STEROID REPLACEMENT ON IMMUNOREACTIVE GONADOTROPIN-RELEASING HORMONE IN THE HYPOTHALAMUS AND PREOPTIC AREA
EFFECT OF CASTRATION AND STEROID REPLACEMENT ON IMMUNOREACTIVE GONADOTROPIN-RELEASING HORMONE IN THE HYPOTHALAMUS AND PREOPTIC AREA
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DOI：
10.1210/endo-106-5-1442
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发表时间：
1980-01-01
期刊：
影响因子：
4.8
通讯作者：
GROSS, DS
中科院分区：
文献类型：
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作者：
GROSS, DS
本文观察了雄性大鼠和小鼠单独去势、去势后给予丙酸睾酮或雌性大鼠给予苯甲酸雌二醇（EB）对正中隆起和终板血管器（OVLT）内促性腺激素释放激素（gonadoliberin，GnRH）含量和分布的影响。用免疫细胞化学的过氧化物酶-抗过氧化物酶法，用合成GnRH与牛血清白蛋白结合的抗血清标记标本。去势后5周，雌雄大鼠和小鼠正中隆起中GnRH免疫反应性的含量明显减少。GnRH贮量的减少在正中隆起中部和尾部的轴突和神经末梢中最为明显。性腺类固醇替代治疗逆转了去势诱导的GnRH含量减少。EB未能完全恢复GnRH在正中隆起的卵巢切除小鼠的控制水平，但在其他方面没有性别或物种的替代治疗的有效性差异。所有实验处理对与OVLT相关的GnRH分布或含量均无任何影响。显然，在去除性腺类固醇的负反馈作用后，在正中隆起和丙酸睾酮结束的GnRH产生神经元的分泌活性水平增加，在去卵巢的雄性和去卵巢的雌性中，EB使这些神经元的分泌速率升高到与完整对照动物相似的水平。终止于OVLT的GnRH神经元可能不直接参与促性腺激素分泌的负反馈控制。
The effect of castration alone or castration and subsequent administration of testosterone propionate to males or estradiol benzoate (EB) to females on the content and distribution of gonadotropin-releasing hormone (gonadoliberin, GnRH) in the median eminence and organum vasculosum of the lamina terminalis (OVLT) was studied in rats and mice. Specimens were labeled with the peroxidase-antiperoxidase method of immunocytochemistry using an antiserum to synthetic GnRH conjugated to bovine serum albumin. Five wk after castration, the content of immunoreactive GnRH in the median eminence of rats and mice of both sexes was markedly depleted. The reduction in GnRH stores was most obvious in axons and nerve endings in the middle and caudal regions of the median eminence. Replacement therapy with gonadal steroids reversed the castration-induced reduction in GnRH content. EB failed to completely restore GnRH in the median eminence of ovariectomized mice to control levels, but otherwise there were no differences in the effectiveness of replacement therapy with respect to sex or species. None of the experimental treatments had any effect on the distribution or content of GnRH associated with the OVLT. Apparently after removal of the negative feedback action of gonadal steroids, there is an increased level of secretory activity by GnRH-producing neurons ending in the median eminence and testosterone propionate in orchidectomized males and EB in ovariectomized females return the elevated secretory rate of such neurons to levels similar to those in intact control animals. GnRH neurons ending in the OVLT may not be directly involved in the negative feedback control of gonadotropin secretion.