Control of T lymphocyte morphology by the GTPase Rho.
Control of T lymphocyte morphology by the GTPase Rho.
复制标题
GTPase Rho对T淋巴细胞形态的控制。
DOI：
10.1186/1471-2121-4-2
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发表时间：
2003-02-24
期刊：
影响因子：
--
通讯作者：
McIntyre, BW
中科院分区：
文献类型：
--
作者：
Woodside, DG;Wooten, DK;Teague, TK;Miyamoto, YJ;Caudell, EG;Udagawa, T;Andruss, BF;McIntyre, BW
Rho家族GT3对肌动蛋白细胞骨架的调节支配着多种细胞反应。在这份报告中，我们分析了GTdR Rho在维持T淋巴细胞肌动蛋白细胞骨架中的作用。人T淋巴细胞系HPB-ALL中GTdR Rho的失活不会抑制与整合素β1底物纤连蛋白的组成性高粘附。然而，它确实导致在底物VCAM-1、Fn和β1整合素特异性mAb上的指状树突状突起的异常延伸。延时视频显微镜表明，C3诱导的扩展主要是一个改变伪足伸长，而不是收缩的结果。一旦星状伪足伸展，没有一个收缩，细胞变得完全不动。C3处理的细胞缺乏丝状结构，树突状突起富含丝状肌动蛋白。RhoA在未处理的HPB-ALL细胞中的免疫定位在纤连蛋白上扩散，表现出伪足内的弥漫性染色模式。在C3处理的细胞中，RhoA的簇是明显的，并定位在改变的扩展内。GT3 Rho积极参与T淋巴细胞形态和运动的调节。
Rho family GTPase regulation of the actin cytoskeleton governs a variety of cell responses. In this report, we have analyzed the role of the GTPase Rho in maintenance of the T lymphocyte actin cytoskeleton. Inactivation of the GTPase Rho in the human T lymphocytic cell line HPB-ALL does not inhibit constitutively high adhesion to the integrin β1 substrate fibronectin. It did however result in the aberrant extension of finger-like dendritic processes on the substrates VCAM-1, Fn, and mAb specific to β1 integrins. Time-lapse video microscopy demonstrated that C3 induced extensions were primarily the result of an altered pseudopod elongation rather than retraction. Once the stellate pseudopodia extended, none retracted, and cells became completely immobile. Filipodial structures were absent and the dendritic-like processes in C3 treated cells were rich in filamentous actin. Immunolocalization of RhoA in untreated HPB-ALL cells spreading on fibronectin demonstrated a diffuse staining pattern within the pseudopodia. In C3 treated cells, clusters of RhoA were pronounced and localized within the altered extensions. GTPase Rho is actively involved in the regulation of T lymphocyte morphology and motility.
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影响因子：
5.3
作者：
Kozma, R;Sarner, S;Lim, L
通讯作者：
Lim, L
影响因子：
64.8
作者：
Monks, CRF;Freiberg, BA;Kupfer, A
通讯作者：
Kupfer, A
影响因子：
56.9
作者：
Laudanna, C;Campbell, JJ;Butcher, EC
通讯作者：
Butcher, EC
DOI：
10.1084/jem.179.4.1307
发表时间：
1994-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Faull RJ;Kovach NL;Harlan JM;Ginsberg MH
通讯作者：
Ginsberg MH
影响因子：
3.5
作者：
AEPFELBACHER, M
通讯作者：
AEPFELBACHER, M