Membrane-associated cathepsin L: a role in metastasis of melanomas.
Membrane-associated cathepsin L: a role in metastasis of melanomas.
复制标题
膜相关组织蛋白酶 L：在黑色素瘤转移中的作用。
DOI：
10.1016/0006-291x(89)91755-5
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Sloane,BF
中科院分区：
文献类型：
--
作者：
Rozhin,J;Wade,RL;Honn,KV;Sloane,BF
经过连续差速和 Percoll 密度梯度离心后，在小鼠肝脏、小鼠 B16 无黑色素瘤和人 A2058 黑色素瘤中检查了组织蛋白酶 L 的亚细胞分布，组织蛋白酶 L 是转化的 orras 转染的成纤维细胞释放的主要蛋白质。在小鼠和人类黑色素瘤中，组织蛋白酶 L 的活性在质膜部分中富集；这些级分中的组织蛋白酶 L 为天然形式和酸激活形式。对来自“低”和“高”转移潜力的 B16 黑色素瘤亚群的质膜组分进行了组织蛋白酶 L 和热稳定内源抑制剂的活性测定。在“高”转移潜力的亚群中，组织蛋白酶 L 的相对比活性高出 7 倍，而半胱氨酸蛋白酶抑制活性低 5 倍。由于组织蛋白酶 L 可以降解完整的基底膜，因此这种膜相关的组织蛋白酶 L 很可能有助于黑色素瘤的转移扩散。
Subcellular distribution of cathepsin L, the major protein released by transformed orrastransfected fibroblasts, was examined in murine liver, murine B16 amelanotic melanoma and human A2058 melanoma after sequential differential and Percoll density gradient centrifugation. In both murine and human melanomas, cathepsin L activity was found to be enriched in plasma membrane fractions; cathepsin L in these fractions was in both native and acid activatable forms. Plasma membrane fractions from B16 melanoma subpopulations of “low” and “high” metastatic potential were assayed for activity of cathepsin L and of heat stable endogenous inhibitors. The relative specific activity of cathepsin L was 7-fold greater in the subpopulation of “high” metastatic potential, whereas cysteine proteinase inhibitory activity was 5-fold less. Since cathepsin L can degrade intact basement membrane, this membrane-associated cathepsin L may well contribute to metastatic spread of melanomas.