Increased expression of tetrodotoxin-resistant sodium channels Nav1.8 and Nav1.9 within dorsal root ganglia in a rat model of bone cancer pain
Increased expression of tetrodotoxin-resistant sodium channels Nav1.8 and Nav1.9 within dorsal root ganglia in a rat model of bone cancer pain
复制标题
DOI：
10.1016/j.neulet.2012.01.069
复制
发表时间：
2012-03-23
影响因子：
2.5
通讯作者：
Mi, Weidong
中科院分区：
文献类型：
--
作者：
Qiu, Fang;Jiang, Yuge;Mi, Weidong
为了了解癌症引起的骨痛的潜在机制，我们研究了骨癌疼痛动物模型中背根神经节（DRG）神经元中两种河豚毒素抗性钠通道Nav1.8（SNS/PN 3）和Nav1.9（SNS 2/NaN）的存在。将36只雌性SD大鼠随机分为3组：假手术组（Sham）、7 d后处死荷瘤动物组（C7）和14 d后处死荷瘤动物组（C14）。建立骨癌痛模型后，采用行为学方法分别测定机械痛敏和热痛敏的缩足阈。采用实时定量RT-PCR、Western blot和免疫荧光法检测同侧腰4-5背根神经节中Nav1.8和Nav1.9的mRNA和蛋白表达。与Sham组相比，04组机械痛敏和热痛敏的PWF均显著降低（P
In attempt to understand the underlying mechanisms of cancer-induced bone pain, we investigated the presence of two tetrodotoxin-resistant sodium channels, Nav1.8 (SNS/PN3) and Nav1.9 (SNS2/NaN), in dorsal root ganglia (DRG) neurons in an animal model of bone cancer pain. Thirty-six female Sprague-Dawley rats were randomized into three groups: Sham operation group (Sham), cancer-bearing animals killed after 7 days (C7) and cancer-bearing animals killed after 14 days group (C14). After establishment of bone cancer pain model, behavioral tests were carried out to determine the paw withdrawal threshold (PWT) of mechanical and thermal hyperalgesia, respectively. Real-time RT-PCR, Western bolt and Immunofluorescence were used to determine the mRNA and protein expression of Nav1.8 and Nav1.9 in ipsilateral lumbar 4-5 DRG. Compared to Sham group, PWF of mechanical and thermal hyperalgesia in 04 group displayed a significant decrease (P