Supercritical fluid chromatography with post‐column addition of supporting electrolyte solution for electrochemical determination of tocopherol and tocotrienol isomers
Supercritical fluid chromatography with post‐column addition of supporting electrolyte solution for electrochemical determination of tocopherol and tocotrienol isomers
复制标题
柱后添加支持电解质溶液的超临界流体色谱法用于电化学测定生育酚和生育三烯酚异构体
DOI：
10.1002/jssc.202100923
复制
发表时间：
2022
影响因子：
3.1
通讯作者：
Hakamata Hideki
中科院分区：
文献类型：
--
作者：
Yamamoto Kazuhiro;Nishimura Takuma;Machida Koichi;Kotani Akira;Hakamata Hideki
建立了超临界流体色谱-电化学检测法同时测定生育酚和生育三烯酚异构体的方法。将具有电化学检测系统的超临界流体色谱与另外的泵连接以产生添加支持电解质溶液的流动路径。支持电解质溶液以柱后方式与移动的相混合，从而能够独立控制分离和检测。优化测定条件后，以超临界CO2-甲醇（99：1，v/v）为移动的相，氰丙基柱（4.6 mm内径× 250 mm长，5 μm），在30 min内分离维生素E异构体和内标物（2，2，5，7，8-五甲基-6-羟基苯并二氢吡喃）。以含1.0 mol/L乙酸铵的甲醇为支持电解质，在+0.8 V电位下进行电化学检测。该方法线性范围为5-100 μg/mL，重复性好，相对标准偏差小于2.5%（n= 6），适用于营养补充剂中生育酚和生育三烯酚异构体的测定。
A supercritical fluid chromatography with electrochemical detection system was developed for the simultaneous determination of tocopherol and tocotrienol isomers. The supercritical fluid chromatography with electrochemical detection system was connected with an additional pump to create a flow path to add a supporting electrolyte solution. The supporting electrolyte solution was mixed with a mobile phase in a post‐column fashion, enabling the independent control of the separation and detection. After optimization of the measurement conditions, vitamin E isomers and an internal standard substance (2,2,5,7,8‐pentamethyl‐6‐hydroxychroman) were separated within 30 min using a mixture of supercritical carbon dioxide and methanol (99:1, v/v) as a mobile phase and a cyanopropyl column (4.6 mm inner diameter × 250 mm length, 5 μm). For the electrochemical detection, methanol containing 1.0 mol/L ammonium acetate was used as a supporting electrolyte solution, and the applied potential was set at +0.8 V. This analytical method showed good linearity (5–100 μg/mL) and repeatability (less than 2.5% relative standard deviation,n= 6) and was applicable to the determination of tocopherol and tocotrienol isomers in nutrition supplements.