Protective effects of naringin against paraquat-induced acute lung injury and pulmonary fibrosis in mice
Protective effects of naringin against paraquat-induced acute lung injury and pulmonary fibrosis in mice
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柚皮苷对百草枯所致小鼠急性肺损伤和肺纤维化的保护作用
DOI：
10.1016/j.fct.2013.04.024
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发表时间：
2013-08-01
影响因子：
4.3
通讯作者：
Li, Pei-bo
中科院分区：
文献类型：
--
作者：
Chen, Yan;Nie, Yi-chu;Li, Pei-bo
本研究评价了柚皮苷对百草枯(PQ)诱导的小鼠急性肺损伤(ALI)和肺纤维化的保护作用。通过一次腹腔注射PQ来测试小鼠抵抗PQ中毒的存活概率。结果表明，PQ单独染毒(7d内50 mg/kg)小鼠的存活率明显低于NAC或柚皮苷每日染毒小鼠。此外，在PQ(30 mg/kg，ip)前3d每日给小鼠注射生理盐水、NAC或柚皮苷，以检测对PQ诱导的ALL的保护作用。结果表明，柚皮苷可剂量依赖性地减轻PQ染毒8h所致的白细胞浸润增加和肿瘤坏死因子-α、转化生长因子-β1的过度表达。给小鼠灌胃PQ(20 mg/kg，ip)，然后每天给生理盐水、NAC或柚皮苷灌胃21d，观察对PQ诱导的肺纤维化的保护作用。结果表明，60和120 mg/kg的柚皮苷可显著降低PQ诱导的肺组织中肿瘤坏死因子-α、转化生长因子-β1、基质金属蛋白酶-9和基质金属蛋白酶-1的表达，降低肺组织丙二醛和羟脯氨酸的含量，减少肺纤维化的形成，提高肺组织中超氧化物歧化酶、谷胱甘肽过氧化物酶和HO-1的活性。这些结果表明，柚皮苷对PQ诱导的ALL和肺纤维化有有效的保护作用。(C)2013爱思唯尔有限公司。保留所有权利。
The present study evaluates protective effects of naringin against paraquat (PQ)-induced acute lung injury (ALI) and pulmonary fibrosis in mice. Survival probability against PQ intoxication was tested by a single intraperitoneal injection of PQ. Results showed that survival rates of mice exposed to PQ only (50 mg/kg within 7 days) were much lower than that in mice daily treatment with NAC or naringin. Moreover, protection against PQ-induced ALL was tested by daily pretreatment mice with saline, NAC or naringin for 3 days before PQ (30 mg/kg, i.p.). Results showed that increase in leukocytes infiltration and overexpressions of TNF-alpha and TGF-beta 1 caused by 8 h of PQ exposure were dose-dependently ameliorated by naringin. Furthermore, protection against PQ-induced pulmonary fibrosis was tested by pretreatment mice with PQ (20 mg/kg, i.p.), and then daily administration with saline, NAC or naringin for prolonged 21 days. Results showed that naringin of 60 and 120 mg/kg significantly reduced PQ-induced upregulations of TNF-alpha, TGF-beta 1, MMP-9 and TIMP-1, levels of pulmonary malonaldehyde and hydroxyproline, as well as pulmonary fibrosis deposition, while increased activities of SOD, GSH-Px and HO-1. These results indicated that naringin had effective protection against PQ-induced ALL and pulmonary fibrosis. (C) 2013 Elsevier Ltd. All rights reserved.