FLUORESCENCE INSITU HYBRIDIZATION WITH HUMAN CHROMOSOME-SPECIFIC LIBRARIES - DETECTION OF TRISOMY-21 AND TRANSLOCATIONS OF CHROMOSOME-4
FLUORESCENCE INSITU HYBRIDIZATION WITH HUMAN CHROMOSOME-SPECIFIC LIBRARIES - DETECTION OF TRISOMY-21 AND TRANSLOCATIONS OF CHROMOSOME-4
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DOI：
10.1073/pnas.85.23.9138
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发表时间：
1988-12-01
影响因子：
11.1
通讯作者：
GRAY, J
中科院分区：
文献类型：
--
作者：
PINKEL, D;LANDEGENT, J;GRAY, J
通过与整个染色体特异性DNA文库进行原位杂交，可以在中期铺展和间期核中特异性地染色染色体。未标记的人基因组DNA用于抑制文库中与多条染色体结合的序列的杂交。目标染色体可以被制成比其他染色体每单位长度至少亮20倍。利用该技术可在中期铺展和间期核中检测到21三体和涉及4号染色体的易位。
Chromosomes can be specifically stained in metaphase spreads and interphase nuclei by in situ hybridization with entire chromosome-specific DNA libraries. Unlabeled human genomic DNA is used to inhibit the hybridization of sequences in the library that bind to multiple chromosomes. The target chromosome can be made at least 20 times brighter per unit length than the others. Trisomy 21 and translocations involving chromosome 4 can be detected in metaphase spreads and interphase nuclei by using this technique.