Cell division in bacillus subtilis: FtsZ and FtsA association is Z-ring independent, and FtsA is required for efficient midcell Z-ring assembly
Cell division in bacillus subtilis: FtsZ and FtsA association is Z-ring independent, and FtsA is required for efficient midcell Z-ring assembly
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DOI：
10.1128/jb.187.18.6536-6544.2005
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发表时间：
2005-09-01
影响因子：
3.2
通讯作者：
Harry, EJ
中科院分区：
文献类型：
--
作者：
Jensen, SO;Thompson, LS;Harry, EJ
细菌细胞分裂的最早阶段是在中细胞的分裂位点组装Z环。其他分裂蛋白也被募集到这个位点来协调分隔过程。FtsA是与FtsZ直接相互作用的胞质分裂蛋白。它的功能仍然未知。一般认为，FtsA定位到分裂位点后立即发生Z-环形成或伴随着它，FtsA是负责招聘后来组装膜结合分裂蛋白的分裂位点，在这里，我们报告的发展，在体内化学交联试验，以检查FtsZ和FtsA之间的关联在枯草芽孢杆菌细胞。我们随后在同步细胞周期中使用该测定来显示这两种蛋白质可以在Z环形成之前相互作用。我们进一步表明，在一个B。在含有ftsA缺失的枯草芽孢杆菌菌株中，FtsZ沿着丝状体以规则的间隔沿着定位，但大多数Z环是异常的。因此，这种生物体中的FtsA对于有效形成功能性Z环至关重要。这是第一次报告的异常Z环形成的损失造成的一个单一的分隔蛋白。这些结果表明，在这种生物体中，也许还有其他生物体，FtsA通过确保Z环的正确形成来确保膜结合分裂蛋白的募集。
The earliest stage in cell division in bacteria is the assembly of a Z ring at the division site at midcell. Other division proteins are also recruited to this site to orchestrate the septation process. FtsA is a cytosolic division protein that interacts directly with FtsZ. Its function remains unknown. It is generally believed that FtsA localization to the division site occurs immediately after Z-ring formation or concomitantly with it and that FtsA is responsible for recruiting the later-assembling membrane-bound division proteins to the division site, Here, we report the development of an in vivo chemical cross-linking assay to examine the association between FtsZ and FtsA in Bacillus subtilis cells. We subsequently use this assay in a synchronous cell cycle to show that these two proteins can interact prior to Z-ring formation. We further show that in a B. subtilis strain containing an ftsA deletion, FtsZ localized at regular intervals along the filament but the majority of Z rings were abnormal. FtsA in this organism is therefore critical for the efficient formation of functional Z rings. This is the first report of abnormal Z-ring formation resulting from the loss of a single septation protein. These results suggest that in this organism, and perhaps others, FtsA ensures recruitment of the membrane-bound division proteins by ensuring correct formation of the Z ring.