Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms
Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms
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芥子碱对羟基自由基诱导的间充质干细胞损伤的保护作用及其可能机制
DOI：
10.1248/cpb.c15-00850
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发表时间：
2016-04-01
影响因子：
1.7
通讯作者：
Wei, Gang
中科院分区：
文献类型：
--
作者：
Li, Xican;Han, Lu;Wei, Gang
作为一种存在于十字花科植物中的酚性生物碱，本研究观察到Sinoprotein对间充质干细胞（MSCs）免受中心点OH诱导的损伤具有保护作用。还发现它能防止DNA损伤，消除各种自由基（中心点OH，中心点O-2（-），2，2 ′-连氮基-双（3-乙基苯并噻唑啉-6-磺酸二铵盐）（ABTS）（+），和1，1-二苯基-2-苦基肼基自由基（DPPIF中心点）），并将Cu 2+还原为Cu+。为进一步探讨其作用机理，采用高效液相色谱-电喷雾离子化（ESI）-串联质谱（HPLC-MS/MS）和高效液相色谱-二极管阵列检测器（DAD）对辛硫磷与DPPH中心点反应的终产物进行了分析。HPLC-ESI-MS/MS分析中的四个分子离子峰（Ink 701、702、703和351）表明自由基加合物形成（RAF）途径;而HPLC-DAD中的红移（λ（max）334 -> 475 nm）表明形成醌作为酚-OH基团的氧化产物。基于这些结果，可以得出结论，（i）Sinoprotein可以有效地防止中心点OH诱导的DNA和MSC损伤;这种保护作用可能为Sinoprotein在MSC移植治疗中的潜在作用提供证据，并且是十字花科植物的有益作用的原因。(ii)Sinoprotein保护中心点OH诱导的氧化损伤的可能机制是清除自由基，其被认为是通过氢原子（H中心点）转移（HAT）（或顺序电子（e）质子转移（SEPT））-> RAF途径。
As a phenolic alkaloid occurring in Cruciferous plants, sinapine was observed to protect mesenchymal stem cells (MSCs) against center dot OH-induced damage in this study. It was also found to prevent DNA from damage, to scavenge various free radicals (center dot OH, center dot O-2(-), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt) (ABTS)(+.)., and 1,1-diphenyl-2-picrylhydrazyl radical (DPPIF center dot)), and to reduce Cu2+ to Cu+. To further explore the mechanism, the end-product of sinapine reaction with DPPH center dot was determined using HPLC-electrospray ionization (ESI)-MS/MS and HPLC-diode array detector (DAD). Four molecular ion peaks (Ink 701, 702, 703, and 351) in HPLC-ESI-MS/MS analysis indicated a radical adduct formation (RAF) pathway; while a bathochromic shift (lambda(max) 334 -> 475 nm) in HPLC-DAD indicated the formation of quinone as the oxidized product of the phenolic-OH group. Based on these results, it may be concluded that, (i) sinapine can effectively protect against center dot OH-induced damage to DNA and MSCs; such protective effect may provide evidence for a potential role for sinapine in MSC transplantation therapy, and be responsible for the beneficial effects of Cruciferous plants. (ii) The possible mechanism for sinapine to protect against center dot OH-induced oxidative damage is radical-scavenging, which is thought to be via hydrogen atom (H center dot) transfer (HAT) (or sequential electron (e) proton transfer (SEPT))-> RAF pathways.