Efficient Production of Triacetic Acid Lactone from Lignocellulose Hydrolysate by Metabolically Engineered Yarrowia lipolytica.
Efficient Production of Triacetic Acid Lactone from Lignocellulose Hydrolysate by Metabolically Engineered Yarrowia lipolytica.
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DOI：
10.1021/acs.jafc.3c06528
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发表时间：
2023-11
影响因子：
6.1
通讯作者：
Huan Liu;Xiaolan Huang;Yangming Liu;Xinyun Jing;Yuchen Ning;Peng Xu;Li Deng;Fang Wang
中科院分区：
文献类型：
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作者：
Huan Liu;Xiaolan Huang;Yangming Liu;Xinyun Jing;Yuchen Ning;Peng Xu;Li Deng;Fang Wang
木质纤维素是一种很有前途的可再生原料，可用于高价值生物化学品的生物生产。木糖代谢途径的低表达是制约酵母高效利用木质纤维素原料的瓶颈。本文中，探索了多种遗传和工艺工程策略以解除解脂耶氏酵母中木糖转化为平台化学品三乙酸内酯（TAL）的瓶颈。我们确定木糖同化产生更多的辅因子NADPH有利于TAL的合成。pH控制提高乙酰辅酶A羧化酶的表达，并产生更多的前体丙二酰辅酶A。结合对脂质合成途径的抑制，纯木糖和富含木糖的麦草水解液分别产生5.03和4.18 g/L的TAL。我们的工作消除了木糖同化途径的瓶颈，并有效地将小麦秸秆水解液升级为TAL，这使我们能够建立一个可持续的产油酵母细胞工厂，以经济高效地从低成本的木质纤维素生产绿色化学品。解脂
Lignocellulose is a promising renewable feedstock for the bioproduction of high-value biochemicals. The poorly expressed xylose catabolic pathway was the bottleneck in the efficient utilization of the lignocellulose feedstock in yeast. Herein, multiple genetic and process engineering strategies were explored to debottleneck the conversion of xylose to the platform chemical triacetic acid lactone (TAL) in Yarrowia lipolytica. We identified that xylose assimilation generating more cofactor NADPH was favorable for the TAL synthesis. pH control improved the expression of acetyl-CoA carboxylase and generated more precursor malonyl-CoA. Combined with the suppression of the lipid synthesis pathway, 5.03 and 4.18 g/L TAL were produced from pure xylose and xylose-rich wheat straw hydrolysate, respectively. Our work removed the bottleneck of the xylose assimilation pathway and effectively upgraded wheat straw hydrolysate to TAL, which enabled us to build a sustainable oleaginous yeast cell factory to cost-efficiently produce green chemicals from low-cost lignocellulose by Y. lipolytica.