Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen
Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen
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通过 T7 噬菌体显示屏探索全氟辛烷磺酸的结合蛋白
DOI：
10.1016/j.bmc.2012.05.016
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Sakaguchi K.
中科院分区：
文献类型：
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作者：
Miyano Y;Tsukuda S;Sakimoto I;Takeuchi R;Shimura S;Takahashi N;Kusayanagi T;Takakusagi Y;Okado M;Matsumoto Y;Takakusagi K;Takeuchi T;Kamisuki S;Nakazaki A;Ohta K;Miura M;Kuramochi K;Mizushina Y;Kobayashi S;Sugawara F;Sakaguchi K.
全氟辛烷磺酸（PFOS）是一种广泛存在于自然界的污染物，对动物有毒。我们创建了一个聚乙二醇聚丙烯酰胺共聚物上的全氟辛烷磺酸类似物和分离的肽，优先结合的全氟辛烷磺酸类似物使用T7噬菌体展示系统。利用生物信息学服务器上的FASTAskan程序进行的生物信息学分析显示，有几种人类蛋白质可能与全氟辛烷磺酸结合。其中，我们证实PFOS和重组可溶形式的单核细胞分化抗原CD 14（sCD 14）的表面等离子体生物传感器之间的结合。此外，全氟辛烷磺酸还可抑制小鼠巨噬细胞RAW 264.7细胞中sCD 14诱导的TNF-α产生。
Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells.