Pre- and postsynaptic distribution of cannabinoid and mu opioid receptors in rat spinal cord
Pre- and postsynaptic distribution of cannabinoid and mu opioid receptors in rat spinal cord
复制标题
DOI：
10.1016/s0006-8993(98)01321-3
复制
发表时间：
1999-03-20
期刊：
影响因子：
2.9
通讯作者：
Herkenham, M
中科院分区：
文献类型：
--
作者：
Hohmann, AG;Briley, EM;Herkenham, M
体外受体结合和定量放射自显影被用来评估大鼠脊髓颈后角大麻素受体的突触前和突触后分布。从C3到T1或T2连续7或8个脊髓节段进行广泛的单侧背根切断术。大麻素和μ阿片受体在中央（C6）脊髓节段的密度进行了评估后2，4，8，和16天的神经根切断术，并与未处理的大鼠进行比较。神经根切断术诱导与脊髓大麻素受体结合的[H-3] CP 55，940的同侧损失约50%，在神经根切断术后8天达到最大。相比之下，[H-3][D-Ala（2）-MePhe（4），Gly-ol（5）]脑啡肽（DAMGO）与μ受体的结合在邻近切片中消耗约60%。相比之下，[H-3] CP 55，940的变化结合对侧的传入神经阻滞在很大程度上是不存在的所有损伤后延迟。这些数据表明，在脊髓节段完全去传入的条件下，颈（C6）背角中约50%的大麻素受体突触前位于初级传入神经的中央末梢上。本研究结果为大麻素受体在脊髓背角的突触前和突触后定位提供了解剖学证据。(C)出版社：Elsevier Science B. V.
In vitro receptor binding and quantitative autoradiography were used to assess the pre- and postsynaptic distribution of cannabinoid receptors in the cervical dorsal horn of the rat spinal cord. An extensive unilateral dorsal rhizotomy was performed across seven or eight successive spinal segments from C3 to T1 or T2. The densities of cannabinoid and mu opioid receptors in the central (C6) spinal segment were assessed 2, 4, 8, and 16 days post rhizotomy and compared with those of untreated rats. Rhizotomy induced approximately a 50% ipsilateral loss in the [H-3]CP55,940 binding to spinal cannabinoid receptors that was maximal at 8 days post-rhizotomy. By comparison, the binding of [H-3][D-Ala(2)-MePhe(4),Gly-ol(5)]enkephalin (DAMGO) to mu receptors was depleted approximately 60% in near-adjacent sections. By contrast, changes in [H-3]CP55,940 binding contralateral to the deafferentation were largely absent at all post-lesion delays. These data suggest that under conditions in which a spinal segment is completely deafferented, approximately 50% of cannabinoid receptors in the cervical (C6) dorsal horn reside presynaptically on central terminals of primary afferents. The present data provide anatomical evidence for presynaptic as well as postsynaptic localization of cannabinoid receptors in the spinal dorsal hem. (C) 1999 Published by Elsevier Science B.V.