VEGF expression and cell apoptosis in NOD mouse retina
VEGF expression and cell apoptosis in NOD mouse retina
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DOI：
10.3980/j.issn.2222-3959.2010.03.10
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发表时间：
2010-09-18
影响因子：
1.4
通讯作者：
Sun, Shu-Guang
中科院分区：
文献类型：
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作者：
Li, Cai-Rui;Sun, Shu-Guang
目的：方法：将实验动物分为非糖尿病组、对照组（2、4、6、8、12周亚组，n=30）和糖尿病组（2、4、6、8、12周亚组，n = 30）。采用酶联免疫吸附法（ELISA）检测血清和视网膜中VEGF的水平。结果：与对照组相比，NOD组血清和视网膜中VEGF水平均显著升高（12周：4.9 ± 0.41 μ g/g vs 0.19 ± 0.1 μ g/g，P
AIM: To investigate retinal vascular endothelial growth factor (VEGF) level and retinal cells apoptosis in the early stage of diabetic NOD mouse retina.METHODS: Animals were divided into non-diabetes group, (control) (2-, 4-, 6-, 8- and 12-week sub-groups, n=30) and diabetes group (2-, 4, 6-, 8- and 12-week sub-groups, n = 30). Enzyme-linked immunosorbent assay (ELISA) was performed to detect VEGF level in both serum and retina. Transmission electron microscope method was used to examine retinal cell apoptosis.RESULTS: Compared with the control group, VEGF levels in serum and retina were increased significantly in the NOD group (12 weeks: 4.9 +/- 0.41 mu g/g vs 0.19 +/- 0.1 mu g/g in serum sample, P