I-Optimal Design of Hierarchical 3D Scaffolds Produced by Combining Additive Manufacturing and Thermally Induced Phase Separation.
I-Optimal Design of Hierarchical 3D Scaffolds Produced by Combining Additive Manufacturing and Thermally Induced Phase Separation.
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DOI：
10.1021/acsabm.8b00534
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发表时间：
2018-12
影响因子：
4.7
通讯作者：
A. Yousefi;Junyi Liu;Riley Sheppard;S. Koo;J. Silverstein;Jing Zhang;P. James
中科院分区：
文献类型：
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作者：
A. Yousefi;Junyi Liu;Riley Sheppard;S. Koo;J. Silverstein;Jing Zhang;P. James
氧气、营养物质和代谢废物运输的限制对临床相关尺寸的生物工程骨的开发提出了挑战。本文报道了由聚乳酸-乙醇酸和纳米羟基磷灰石（PLGA/nHA）制成的分层大孔/微孔支架的设计和表征。这些支架是通过结合增材制造（AM）和热致相分离（TIPS）技术来生产的。通过将多孔 3D 绘制的聚乙二醇 (PEG) 嵌入 PLGA/nHA/1,4-二恶烷或 PLGA/1,4-二恶烷溶液中，然后使用去离子 (DI) 水提取 PEG，生成直径为 ~300 μm、~380 μm 和 ~460 μm 的大通道。我们使用 I 最优实验设计 (DoE) 和响应面分析（JMP® 软件）将三种响应（支架厚度、孔隙率和模量）与影响支架宏观/微观结构的四个实验因素（例如 PEG 链直径、PLGA 浓度、nHA 含量和 TIPS 温度）联系起来。我们的结果表明，约 380 μm 的 PEG 链直径、约 10% w/v 的 PLGA 浓度、约 10% w/w 的 nHA 含量和 -10°C 左右的 TIPS 温度可以生成孔隙率约 90% 且模量超过 4 MPa 的支架。本文介绍了这些支架的 I 优化设计步骤，并报告了它们的宏观/微观结构，并使用扫描电子显微镜 (SEM) 和微型计算机断层扫描 (micro-CT) 进行了表征。
The limitations in the transport of oxygen, nutrients, and metabolic waste products pose a challenge to the development of bioengineered bone of clinically relevant size. This paper reports the design and characterization of hierarchical macro/microporous scaffolds made of poly(lactic-co-glycolic) acid and nanohydroxyapatite (PLGA/nHA). These scaffolds were produced by combining additive manufacturing (AM) and thermally induced phase separation (TIPS) techniques. Macrochannels with diameters of ~300 μm, ~380 μm, and ~460 μm were generated by embedding porous 3D-plotted polyethylene glycol (PEG) inside PLGA/nHA/1,4-dioxane or PLGA/1,4-dioxane solutions, followed by PEG extraction using deionized (DI) water. We have used an I-optimal design of experiments (DoE) and the response surface analysis (JMP® software) to relate three responses (scaffold thickness, porosity, and modulus) to the four experimental factors affecting the scaffold macro/microstructures (e.g., PEG strand diameter, PLGA concentration, nHA content, and TIPS temperature). Our results indicated that a PEG strand diameter of ~380 μm, a PLGA concentration of ~10% w/v, a nHA content of ~10% w/w, and a TIPS temperature around -10°C could generate scaffolds with a porosity of ~90% and a modulus exceeding 4 MPa. This paper presents the steps for the I-optimal design of these scaffolds and reports on their macro/microstructures, characterized using scanning electron microscopy (SEM) and micro-computed tomography (micro-CT).