Efficient plant regeneration from cotyledonary node explants of Cucumis melo L.
Efficient plant regeneration from cotyledonary node explants of Cucumis melo L.
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发表时间：
2011
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通讯作者：
Huijun Zhang;G. Peng;Luan Feishi
中科院分区：
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作者：
Huijun Zhang;G. Peng;Luan Feishi
以甜瓜自交系‘CM-15’和‘CM-23’的子叶节为外植体，建立了高效的再生体系。在Murashige和Skoog(MS)附加不同组合的6-苄基氨基嘌呤(BA)和吲哚-3-乙酸(IAA)的固体培养基上诱导不定芽。‘CM-15’和‘CM-23’外植体在MS+BA(1.5 mg/L)和IAA(0.1 mg/L)、MS+BA(1.5 mg/L)和IAA(0.5 mg/L)的培养基上诱导效果最好，其中自交系CM-15的再生率最高。将丛生芽转移到附加BA0.05 mg/L的MS上，可使大部分不定芽分化为发育良好的不定芽。再生芽在不含植物生长调节剂(PGRs)的半强度MS培养基上生根。生根苗在土壤中生根，成功率为100%。这种高频植株再生系统为甜瓜遗传转化提供了改进的技术。关键词：甜瓜，子叶节，芽再生，植株再生。
An efficient regeneration system was developed using cotyledonary node of two inbred line of melon, Cucumis melo 'CM-15' and 'CM-23'. The induction of shoots was achieved on Murashige and Skoog (MS) solid medium supplemented with different combinations of 6-benzylaminopurine (BA) and indole- 3-acetic acid (IAA). The best shoot induction was observed when the explants were cultured on MS medium supplemented with BA (1.5 mg/L) and IAA (0.1 mg/L), and on MS medium supplemented with BA (1.5 mg/L) and IAA (0.5 mg/L) for 'CM-15' and 'CM-23', and inbred line CM-15 had the best regeneration rate. The shoot clumps were transferred to MS with BA (0.05 mg/L), resulting to the differentiation of most of the shoots initially into well developed shoots. Regenerated shoots were rooted on half-strength MS medium without plant growth regulators (PGRs). The rooted plants were established in soil with 100% success rate. This high frequency plant regeneration system provides improved technology to assist in genetic transformation of melon. Key words : Cucumis Melo L., cotyledonary node, shoots regeneration, plant regeneration.