Germ cell-Sertoli cell interactions: the effect of testicular maturation on the synthesis of cyclic protein-2 by rat Sertoli cells.
Germ cell-Sertoli cell interactions: the effect of testicular maturation on the synthesis of cyclic protein-2 by rat Sertoli cells.
复制标题
生殖细胞-支持细胞相互作用：睾丸成熟对大鼠支持细胞合成环状蛋白2的影响。
DOI：
10.1095/biolreprod43.1.25
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发表时间：
1990
影响因子：
3.6
通讯作者：
Wright,WW
中科院分区：
文献类型：
--
作者：
Zabludoff,SD;Karzai,AW;Wright,WW
环蛋白-2（CP-2）是由成熟的大鼠支持细胞在第VI和第VII阶段的曲细精管内以阶段特异性方式合成的。为了确定睾丸成熟如何影响CP-2的合成，我们培养了20厘米的小管，涵盖了17，35，45和75天大的大鼠周期的所有阶段。CP-2合成的最大增加被发现发生在35和45天之间，超过了转铁蛋白和硫酸化糖蛋白（SGP）-2。此外，二维凝胶分析表明，CP-2的分泌从35天增加到45天，其程度大于SGP-1和SGP-2以及转铁蛋白的分泌。生化分析还表明，CP-2的合成是阶段特异性的45天。免疫细胞化学扩大了这些观察结果; CP-2在7 - 35天龄的Sertoli细胞中没有检测到。然而，在第36天，CP-2检测支持细胞在第VI和VII阶段小管，但不是在任何其他阶段。第VI-VII期肾小管中CP-2浓度增加38天，但此后无变化。最后，我们用免疫细胞化学方法检测了近曲肾小管CP-2浓度的年龄相关变化。该分析显示，在1周时，CP-2存在于除被膜下区域外的所有近端小管中;然而，到14天时，在所有近端小管中检测到CP-2。支持细胞和近端小管细胞的比较表明CP-2含量是由细胞的成熟度而不是动物的年龄决定的。
Cyclic Protein-2 (CP-2) is synthesized in a stage-specific manner by mature rat Sertoli cells within stage VI and VII seminiferous tubules. To determine how testicular maturation affects CP-2 synthesis, we cultured 20 cm of tubules encompassing all stages of the cycle from rats 17, 35, 45, and 75 days old. The greatest increase in CP-2 synthesis was found to occur between 35 and 45 days and exceeded that observed for transferrin and sulfated glycoprotein (SGP)-2. Additionally, two-dimensional gel analysis indicated that secretion of CP-2 increased from 35 to 45 days to a greater extent than the secretion of SGP-1 and SGP-2 and transferrin. Biochemical analysis also demonstrated that CP-2 synthesis was stage-specific by 45 days. Immunocytochemistry expanded these observations; CP-2 was not detected in 7−35-day-old Sertoli cells. However, at 36 days, CP-2 was detected in Sertoli cells in stage VI and VII tubules but not at any other stage. CP-2 concentration in stage VI-VII tubules was increased by 38 days, but was unchanged thereafter. Finally, we immunocytochemically examined age-related changes in CP-2 concentration of the proximal convoluted kidney tubule. This analysis revealed that, at 1 wk, CP-2 was present in all proximal tubules except those in the subcapsular area; however, by 14 days, CP-2 was detected in all proximal tubules. This comparison of Sertoli cells and proximal tubule cells indicates that CP-2 content is determined by the maturity of a cell and not by the age of the animal.