Early Time Course of Akt Phosphorylation after Endurance and Resistance Exercise
Early Time Course of Akt Phosphorylation after Endurance and Resistance Exercise
复制标题
DOI：
10.1249/mss.0b013e3181d964e4
复制
发表时间：
2010-10-01
期刊：
影响因子：
--
通讯作者：
Coffey, Vernon G.
中科院分区：
文献类型：
--
作者：
Camera, Donny M.;Edge, Johann;Coffey, Vernon G.
题名/责任者：The First of First，by J.耐力耐力运动后Akt蛋白磷酸化的早期时程变化地中海医院。SCI。《体育运动》，第42卷，第10期，第1843-1852页，2010年。目的：本研究的目的是确定与阻力和耐力运动相关的不同收缩活动后运动诱导信号的早期时间进程。方法：将16名男性受试者随机分为两组：自行车组(n=8，60min，60min)和阻力训练组(REX组，n=8，伸腿8x5，最大重复次数80%，恢复3min)。在运动前、运动后即刻、运动后15、30、60min分别于安静状态下取股外侧肌连续取材，以确定运动后的早期信号反应。结果：静息时Akt(Thr308/Ser473)和mTOR(Ser2448)磷酸化水平在运动后30-60min达到峰值(P<；0.05)。P70S6K(Thr389)和4E-BP1(Thr37/46)的磷酸化模式也相似，但REX和CyC的影响更大(P<；0.05)。然而，AMPK(Thr172)磷酸化只有在CyC后才显著升高(P<；0.05)，我们观察到对糖原合成酶(Ser641)和AS160磷酸化的不同反应，在CyC后增强，而REX没有(P<；0.05)。结论：在不同的收缩刺激后，Akt-mTOR-S6K的磷酸化在最初60min的恢复期表现出相似的时间进程。相反，促进葡萄糖转运和糖原合成的蛋白质的磷酸化状态只有在耐力运动后才会增强。我们的结果表明，耐力和抗阻运动启动了翻译信号，但高负荷、低重复的收缩活动未能促进糖代谢调节通路的磷酸化。
CAMERA, D. M., J. EDGE, M. J. SHORT, J. A. HAWLEY, and V. G. COFFEY. Early Time Course of Akt Phosphorylation after Endurance and Resistance Exercise. Med. Sci. Sports Exerc., Vol. 42, No. 10, pp. 1843-1852, 2010. Purpose: The aim of this study was to determine the early time course of exercise-induced signaling after divergent contractile activity associated with resistance and endurance exercise. Methods: Sixteen male subjects were randomly assigned to either a cycling (CYC; n = 8, 60 min, 70% (V)over dot O-2peak) or resistance (REX; n = 8, 8 x 5 leg extension, 80% one-repetition maximum, 3-min recovery) exercise group. Serial muscle biopsies were obtained from vastus lateralis at rest before, immediately after, and after 15, 30, and 60 min of passive recovery to determine early signaling responses after exercise. Results: There were comparable increases from rest in Akt(Thr308/ Ser473) and mTOR(Ser2448) phosphorylation during the postexercise time course that peaked 30-60 min after both CYC and REX (P < 0.05). There were also similar patterns in p70S6K(Thr389) and 4E-BP1(Thr37/46) phosphorylation, but a greater magnitude of effect was observed for REX and CYC, respectively (P < 0.05). However, AMPK(Thr172) phosphorylation was only significantly elevated after CYC (P < 0.05), and we observed divergent responses for glycogen synthase(Ser641) and AS160 phosphorylation that were enhanced after CYC but not REX (P < 0.05). Conclusions: We show a similar time course for Akt-mTOR-S6K phosphorylation during the initial 60-min recovery period after divergent contractile stimuli. Conversely, enhanced phosphorylation status of proteins that promote glucose transport and glycogen synthesis only occurred after endurance exercise. Our results indicate that endurance and resistance exercise initiate translational signaling, but high-load, low-repetition contractile activity failed to promote phosphorylation of pathways regulating glucose metabolism.