Lactic acid bacteria isolated from canine faeces
Lactic acid bacteria isolated from canine faeces
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DOI：
10.1111/j.1365-2672.2006.02884.x
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发表时间：
2006-07-01
影响因子：
4
通讯作者：
Saris, P. E. J.
中科院分区：
文献类型：
--
作者：
Beasley, S. S.;Manninen, T. J. K.;Saris, P. E. J.
目的：从健康犬粪便中分离乳酸菌并测序。这些菌株中的五个被选择和进一步的特点，以澄清这些菌株作为益生菌的潜力为canine.Methods和结果：LAB被发现在67%（14/21）的犬粪便样本时，平板上的乳酸杆菌选择性培养基不含乙酸。在13个经16 S rRNA基因部分测序鉴定的菌种中，发酵乳杆菌（Lactobacillus fermentum）LAB 8、L.粘膜LAB 12，L. rhamnosus LAB11，L.根据其在粪便中的频率、数量、生长密度、耐酸性和抗菌活性，选择Salivarius LAB 9和Weissella confusa LAB 10作为候选益生菌菌株。测定了这些分离株对14种抗生素的最低抑菌浓度。L. salivariusLAB9、W. confusa LAB 10和L.粘膜LAB 12在pH 2下存活4小时（mLBS），表明对酸性的耐受性，因此具有在犬胃肠道中存活的潜力。结论：从健康犬粪便中分离到13种不同的乳酸菌。五个耐酸和抗菌活性的LAB菌株的能力，以高密度的有氧和厌氧生长被选为候选probiotics.Significance和影响的研究：所选的LAB菌株是第一个主机特定的LAB与抗菌活性分离的犬，可以作为潜在的益生菌犬使用。
Aims: Lactic acid bacteria (LAB) were isolated and sequenced from the faeces of healthy dogs. Five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine.Methods and Results: LAB were found in 67% (14/21) of the canine faeces samples when plated on Lactobacilli Selective Media without acetic acid. Out of 13 species identified with partial 16S rRNA gene sequencing, Lactobacillus fermentum LAB8, L. mucosae LAB12, L. rhamnosus LAB11, L. salivarius LAB9 and Weissella confusa LAB10 were selected as candidate probiotic strains based on their frequency, quantity in faeces, growth density, acid tolerance and antimicrobial activity. The minimal inhibitory concentration values of these isolates were determined for 14 antibiotics. L. salivarius LAB9, W. confusa LAB10 and L. mucosae LAB12 were viable in pH 2 for 4 h (mLBS), indicating tolerance to acidity and thus the potential to survive in gastrointestinal tract of the canine. The LAB8-LAB12 strains showed antimicrobial activity against Micrococcus luteus A1 NCIMB86166.Conclusions: Thirteen different LAB species were found from the faecal microbiota of the healthy canines. Five acid tolerant and antimicrobially active LAB strains with the capacity to grow to high densities both aerobically and anaerobically were chosen to serve as candidate probiotics.Significance and Impact of the Study: The selected LAB strains are among the first host-specific LAB with antimicrobial activity isolated from canines that could serve as potential probiotics for canine use.