Impact of a new refrigerator on the preservation of hepatic grafts.
Impact of a new refrigerator on the preservation of hepatic grafts.
复制标题
新冰箱对肝移植物保存的影响。
DOI：
10.1111/j.1872-034x.2012.00987.x
复制
发表时间：
2012
影响因子：
4.2
通讯作者：
Mami Kanamoto
中科院分区：
文献类型：
--
作者：
Yamada S;Shimada M;Utsunomiya T;Morine Y;Imura S;Ikemoto T;Mori H;Arakawa Y;Kanamoto M;Iwahashi S;Saito Y.;Yu Saito;Iida M;Yu Saito;Ryouichi Tsunedomi;Jun Hanaoka;Hiroki Mori;飯田通久;吉村清;Mami Kanamoto
目的：当前医学移植方法重点解决供体短缺等重大问题。为了克服这些问题，延长器官保存时间已成为一个主要问题。最近开发出一种新型冷藏室，通过频繁感测物料内部和表面的温度，可以将物料内部冷却到所需温度。本研究的目的是评估新型制冷系统在肝脏保存中的有效性。方法：取大鼠肝移植物并分为两组。 A 组由在冷冻威斯康星大学 (UW) 溶液（冰上）中保存 24、72 和 168 小时的移植物组成。 B 组将移植物保存在 4°C 新冰箱中的 UW 溶液中。结果：B 组冷藏 72 h 后释放到废水中的天冬氨酸转氨酶与 A 组相比显着下降（P< 0.05）。 B组氨、乳酸水平显着降低（P< 0.05）。冷藏24小时和72小时后，B组三磷酸腺苷水平较A组显着保持（P< 0.05）。免疫组化显示B组冷藏后血红素加氧酶-1阳性细胞明显增多。结论：这种新型冰箱可以改善肝移植物的保存损伤，可能为肝移植提供一种创新技术。
Aim:Current medical transplantation methods focus on solutions for major problems such as the shortage of donors. To overcome these issues, expanding organ preservation time has become a major concern. A new refrigerating chamber has been recently developed, which can cool the inside of a material to the required temperature by frequently sensing the temperature of both inside and surface of the materials. The purpose of this study is to evaluate the usefulness of a new refrigerating system in hepatic preservation.Methods:The liver grafts were harvested from rats and divided into two groups. Group A consisted of grafts preserved in chilled University of Wisconsin solution (UW) solution (on ice) for 24, 72 and 168 h. Group B consisted of grafts preserved in the UW solution in a new refrigerator at 4°C.Results:In group B, aspartate aminotransferase released into effluent after cold storage for 72 h showed a marked decrease compared to group A (P< 0.05). The levels of ammonia and lactate decreased significantly in group B (P< 0.05). In group B, the levels of adenosine triphosphate were significantly preserved after cold storage for 24 h and 72 h compared to group A (P< 0.05). Immunohistochemistry showed positive cells for heme oxygenase‐1 were significantly increased in group B after cold storage.Conclusion:This new refrigerator can improve preservation injury of hepatic grafts and may provide an innovative technique for liver transplantation.