Staphylococcus aureus Rbf activates biofilm formation in vitro and promotes virulence in a murine foreign body infection model.
Staphylococcus aureus Rbf activates biofilm formation in vitro and promotes virulence in a murine foreign body infection model.
复制标题
金黄色葡萄球菌 Rbf 在体外激活生物膜形成并增强小鼠异物感染模型中的毒力。
DOI：
10.1128/iai.00872-08
复制
发表时间：
2009
影响因子：
3.1
通讯作者：
Lee,ChiaY
中科院分区：
文献类型：
--
作者：
Luong,ThanhT;Lei,MeiG;Lee,ChiaY
We previously identified Rbf as an activator for biofilm formation on polystyrene surfaces inStaphylococcus aureusstrain 8325-4. However, strain 8325-4 contains genetic mutations that may affect biofilm formation. To extend the observation to other strains, we used strain Newman, a weak biofilm producer, and strain UAMS-1, an osteomyelitis clinical strain, in this study. We found that mutations in the chromosomalrbfgene did not affect biofilm formation on polystyrene surfaces in these strains, but transformants of these strains carrying a multiple-copy plasmid containing therbfgene formed stronger biofilms than the wild-type strains and the mutant strains. Using the flow cell method, we found that the chromosomal mutation in therbfgene delayed biofilm formation, whereas strains with a plasmid containing therbfgene accelerated biofilm formation in strains Newman and UAMS-1. These results led us to conclude thatrbfis an activator of biofilm formation in different strains ofS. aureus, although the degree of activation varies among strains. In a murine model of foreign body infection, therbfmutations in strain Newman, but not in strain UAMS-1, reduced the bacterial survival rate in catheter lumen. However, UAMS-1 carrying multiple copies ofrbfin a plasmid increased the bacterial survival rate. The animal studies therefore suggest that Rbf has a role inS. aureusvirulence.
