Dual real-time in vivo monitoring system of the brain-gut axis
Dual real-time in vivo monitoring system of the brain-gut axis
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DOI：
10.1016/j.bbrc.2020.01.090
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发表时间：
2020-04-02
影响因子：
3.1
通讯作者：
Adachi, Takahiro
中科院分区：
文献类型：
--
作者：
Nishimura, Yuya;Fukuda, Yota;Adachi, Takahiro
大脑中枢是认知和情绪之间的相互作用，以及食物和微生物区系的肠道感觉系统，对健康很重要。然而，到目前为止，还没有同时对大脑和肠道进行实时监测的系统。我们试图通过结合活体肠道钙离子成像和脑电，建立一个双重实时监测脑轴的系统。以条件性黄色Cameleon 3.60表达小鼠为实验对象，以辣椒素刺激为阳性对照，同时进行肠道活体成像、迷走神经电生理记录和大脑皮层脑电记录。将辣椒素注入肠腔后，成功地观察到肠神经系统钙信号和大脑皮层局部场电位的同步反应。它们对辣椒素的刺激都有立即的反应。辣椒素引起迷走神经放电频率显著增加，皮层LFP的慢波功率显著降低。辣椒素可诱导肠上皮细胞延迟持续的钙信号，进而抑制肠运动。大脑和肠道的双重实时监测系统能够精确地剖析大脑和肠道随时间的相互作用。(C)2020 Elsevier Inc.保留所有权利。
The brainegut axis which is an interaction between recognition and emotion and the gut sensory system for food and microbiota is important for health. However, there is no real-time monitoring system of the brain and the gut simultaneously so far. We attempted to establish a dual real-time monitoring system for the brainegut axis by a combination of intravital Ca2+ imaging of the gut and electroencephalogram. Using a conditional Yellow Cameleon 3.60 expression mouse line, we performed intravital imaging of the gut, electrophysiological recordings of the vagus nerve, and electroencephalogram recordings of the various cortical regions simultaneously upon capsaicin stimuli as a positive control. Upon capsaicin administration into the small intestinal lumen, a simultaneous response of Ca2+ signal in the enteric nervous system and cortical local field potentials (LFPs) was successfully observed. Both of them responded immediately upon capsaicin stimuli. Capsaicin triggered a significant increase in the frequency of vagus nerve spikes and a significant decrease in the slow-wave power of cortical LFPs. Furthermore, capsaicin induced delayed and sustained Ca2+ signal in intestinal epithelial cells and then suppressed intestinal motility. The dual real-time monitoring system of the brain and the gut enables to dissect the interaction between the brain and the gut over time with precision. (C) 2020 Elsevier Inc. All rights reserved.