Role of ICAM-1 in the aggregation and adhesion of human alveolar macrophages in response to TNF-alpha and INF-gamma.
Role of ICAM-1 in the aggregation and adhesion of human alveolar macrophages in response to TNF-alpha and INF-gamma.
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DOI：
10.1080/09629350120102325
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发表时间：
2001-12
影响因子：
4.6
通讯作者：
Miura, M
中科院分区：
文献类型：
--
作者：
Sasaki, M;Namioka, Y;Ito, T;Izumiyama, N;Fukui, S;Watanabe, A;Kashima, M;Sano, M;Shioya, T;Miura, M
细胞内粘附分子-1（ICAM-1）介导的细胞-细胞粘附被认为在炎症部位起重要作用。最近的证据表明，ICAM-1表面表达的肺泡巨噬细胞增加肺结节病和炎性肉芽肿形成的特点是聚集的巨噬细胞。目前的研究表明，ICAM-1的表达显着升高的肺泡巨噬细胞从结节病患者的肿瘤坏死因子-α（TNF-α）和干扰素-γ（INF-γ）相比，健康对照。用TNF-α和INF-γ处理的肺泡巨噬细胞的聚集和粘附显著增加，而用ICAM-1单克隆抗体预处理的肺泡巨噬细胞的聚集和粘附显著抑制。类似地，在用肝素处理的巨噬细胞中，聚集和粘附被抑制，然后表现出与炎症相关的广泛的生物活性。这些结果表明，响应TNF-α和INF-γ的肺泡巨噬细胞表面ICAM-1的表达在介导聚集和粘附中是重要的。此外，肝素可用于开发纤维化肺病的新型治疗剂。
Intracellular adhesion molecule-1 (ICAM-1)-mediated cell-cell adhesion is thought to play an important role at sites of inflammation. Recent evidence suggests that ICAM-1 surface expression on alveolar macrophages is increased in pulmonary sarcoidosis and that inflammatory granuloma formation is characterized by the aggregation of macrophages. The present study shows that ICAM-1 expression is significantly elevated on alveolar macrophages from patients with sarcoidosis in response to tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (INF-gamma) compared with healthy controls. Aggregation and adhesion were significantly increased in alveolar macrophages treated with TNF-alpha and INF-gamma, and significantly inhibited in those pretreated with a monoclonal antibody to ICAM-1. Similarly, aggregation and adhesion were inhibited in macrophages treated with heparin, which then exhibited a wide range of biological activities relevant to inflammation. These results suggested that the surface expression of ICAM-1 on alveolar macrophages in response to TNF-alpha and INF-gamma is important in mediating aggregation and adhesion. Additionally, heparin may be useful for developing novel therapeutic agents for fibrotic lung disease.